지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 265 - 273 (9page)
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초록· 키워드
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BACKGROUND: Wear debris-induced osteolysis leads to periprosthetic loosening and subsequent prosthetic failure. Since excessive osteoclast formation is closely implicated in periprosthetic osteolysis, identification of agents to suppress osteoclast formation and/or function is crucial for the treatment and prevention of wear particle-induced bone destruction. In this study, we examined the potential effect of pentamidine treatment on titanium (Ti) particle-induced osteolysis, and receptor activator of nuclear factor-jB ligand (RANKL)-induced osteoclastogenesis. METHODS: The effect of pentamidine treatment on bone destruction was examined in Ti particle-induced osteolysis mouse model. Ti particles were implanted onto mouse calvaria, and vehicle or pentamidine was administered for 10 days. Then, calvarial bone tissue was analyzed using micro-computed tomography and histology. We performed in vitro osteoclastogenesis assay using bone marrow-derived macrophages (BMMs) to determine the effect of pentamidine on osteoclast formation. BMMs were treated with 20 ng/mL RANKL and 10 ng/mL macrophage colony-stimulating factor in the presence or absence of pentamidine. Osteoclast differentiation was determined by tartrate-resistant acid phosphatase staining, real-time polymerase chain reaction, and immunofluorescence staining. RESULTS: Pentamidine administration decreased Ti particle-induced osteoclast formation significantly and prevented bone destruction compared to the Ti particle group in vivo. Pentamidine also suppressed RANKL-induced osteoclast differentiation and actin ring formation markedly, and inhibited the expression of nuclear factor of activated T cell c1 and osteoclast-specific genes in vitro. Additionally, pentamidine also attenuated RANKL-mediated phosphorylation of IjBa in BMMs. CONCLUSION: These results indicate that pentamidine is effective in inhibiting osteoclast formation and significantly attenuates wear debris-induced bone loss in mice.
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참고문헌 (29)
참고문헌 신청
Harris WH / 2001 / Wear and periprosthetic osteolysis : the problem / Clin Orthop Relat Res 393:66~70
Holding CA / 2006 / The correlation of RANK, RANKL and TNFalpha expression with bone loss volume and polyethylene wear debris around hip implants / Biomaterials 27:5212~5219
Kim JA / 2015 / Inhibitory effects of triptolide on titanium particle-induced osteolysis and receptor activator of nuclear factor-jB ligand-mediated osteoclast differentiation / Int Orthop 39:173~182
Zhai Z / 2014 / The effect of metallic magnesium degradation products on osteoclast-induced osteolysis and attenuation of NF-jB and NFATc1 signaling / Biomaterials 35:6299~6310
Findlay DM / 2005 / Mechanisms of bone loss in rheumatoid arthritis / Mod Rheumatol 15:232~240
Holding CA / 2006 / The correlation of RANK, RANKL and TNFalpha expression with bone loss volume and polyethylene wear debris around hip implants / Biomaterials 27:5212~5219
Kim JA / 2015 / Inhibitory effects of triptolide on titanium particle-induced osteolysis and receptor activator of nuclear factor-jB ligand-mediated osteoclast differentiation / Int Orthop 39:173~182
Zhai Z / 2014 / The effect of metallic magnesium degradation products on osteoclast-induced osteolysis and attenuation of NF-jB and NFATc1 signaling / Biomaterials 35:6299~6310
Findlay DM / 2005 / Mechanisms of bone loss in rheumatoid arthritis / Mod Rheumatol 15:232~240
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