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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 81 - 93 (13page)
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Cirsium setidens and Silybum marianum are medicinal plants that have been recently recognized as highly valuable sources of herbal drugs in Korea as well as worldwide. However, molecular markers for detecting these species in commercial food products have not yet been reported. In this study, we developed six species-specific primer sets for C. setidens and S. marianum using quantitative real-time PCR (qPCR) analysis, based on the single nucleotide polymorphisms (SNPs) of four chloroplast genes (ndhA, ndhF, rbcL and ycf1). The developed primer pairs showed correlation coefficients for their target species only (R2 > 0.99), and their efficiencies and slopes were in the range of 93－103% and -3.23 to -3.49, respectively. A total of 12 commercial food products including seven for C. setidens and 5 for S. marianum were tested to evaluate the feasibility of the developed primer sets. We found that C. setidens was present in all the seven C. setidens commercial products, while presence of S. marianum was confirmed in four of five S. marianum products. Therefore, the developed species-specific primer sets could prove a useful tool for detecting the presence of C. setidens and S. marianum in commercial food products.
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참고문헌 (30)
참고문헌 신청
Abenavoli, L. / 2018 / Milk thistle (Silybum marianum): A concise overview on its chemistry, pharmacological, and nutraceutical uses in liver diseases / Phytother Res 32:2202~2213
Allmann, M. / 1993 / Polymerase chain reaction (PCR): a possible alternative to immunochemical methods assuring safety and quality of food. Detection of wheat contamination in non-wheat food products / Eur Food Res Technol 196:248~251
안준 / 2018 / Markers for distinguishing Orostachys species by SYBR Green-based real-time PCR and verification of their application in commercial O. japonica food products / Applied Biological Chemistry 61(5):499~508
Arlorio, M. / 2007 / Detection of hazelnut (Corylus spp.) in processed foods using real-time PCR / Food Control 18:140~148
Bahmani, M. / 2015 / (2015)Silybum marianum : beyond hepatoprotection / J Evidence-Based Complementary Altern Med 20:292~301
Allmann, M. / 1993 / Polymerase chain reaction (PCR): a possible alternative to immunochemical methods assuring safety and quality of food. Detection of wheat contamination in non-wheat food products / Eur Food Res Technol 196:248~251
안준 / 2018 / Markers for distinguishing Orostachys species by SYBR Green-based real-time PCR and verification of their application in commercial O. japonica food products / Applied Biological Chemistry 61(5):499~508
Arlorio, M. / 2007 / Detection of hazelnut (Corylus spp.) in processed foods using real-time PCR / Food Control 18:140~148
Bahmani, M. / 2015 / (2015)Silybum marianum : beyond hepatoprotection / J Evidence-Based Complementary Altern Med 20:292~301
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