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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.1
- 수록면
- 138 - 146 (9page)
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Objective: We aimed to determine whether bone marrow-derived mesenchymal stem cells (BDMSCs) effectively attenuate the degeneration of human nucleus pulposus cells (NPCs). Methods: Four NPC lines were obtained from 3 subjects who underwent spinal surgery for cervical disc herniation (n=1) or lumbar disc herniation (n=2). For co-culture wells without contact, BDMSCs and adipose-derived mesenchymal stem cells (ADMSCs) were seeded on tissue culture plates and maintained for 3 days. Senescence-associated β-gal (SA-β-gal) staining was represented as a percentage of the total number of stained cells (%). The cells with intracellular lipid droplets (LDs) were represented as the percentage of the number of cells with LDs. Glycosaminoglycan (GAG) secretion was measured at 450 nm, using a commercial kit, to analyze optical density. Results: The ratio of cells stained with SA-β-gal to the total number of cells reduced signifcantly when co-cultured with BDMSCs and ADMSCs (p<0.001 vs. p<0.001). The proportion of NPCs containing LDs was lower when co-cultured with BDMSCs than with ADMSCs (p<0.001). The optical density related to GAG secretion was lower in BDMSCs and ADMSCs co-cultured with NPCs than in the controls (p<0.001 vs. p<0.001). Conclusion: SA-β-gal staining showed signifcant attenuation of degenerative changes in NPCs co-cultured with BDMSCs. Moreover, the unexpected increase in LDs was signifcantly higher in NPCs co-cultured with ADMSCs than in those co-cultured with BDMSCs. However, GAG secretion was signifcantly decreased in NPCs co-cultured with MSCs.
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참고문헌 (23)
참고문헌 신청
Allon AA / 2009 / Co-culture of adult mesenchymal stem cells and nucleus pulposus cells in bilaminar pellets for intervertebral disc regeneration / SAS J 3:41~49
Banga I / 1975 / Investigations of fluorescent peptides and lipofuscins of human intervertebral disc relating to atherosclerosis / Atherosclerosis 22:533~541
Dickson IR / 1967 / Variations in the protein components of human intervertebral disk with age / Nature 215:52~53
Dimri GP / 1995 / A biomarker that identifies senescent human cells in culture and in aging skin in vivo / Proc Natl Acad Sci U S A 92:9363~9367
Erwin WM / 2014 / The cellular and molecular biology of the intervertebral disc : a clinician's primer / J Can Chiropr Assoc 58:246~257
Banga I / 1975 / Investigations of fluorescent peptides and lipofuscins of human intervertebral disc relating to atherosclerosis / Atherosclerosis 22:533~541
Dickson IR / 1967 / Variations in the protein components of human intervertebral disk with age / Nature 215:52~53
Dimri GP / 1995 / A biomarker that identifies senescent human cells in culture and in aging skin in vivo / Proc Natl Acad Sci U S A 92:9363~9367
Erwin WM / 2014 / The cellular and molecular biology of the intervertebral disc : a clinician's primer / J Can Chiropr Assoc 58:246~257
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