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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.1
- 수록면
- 143 - 154 (12page)
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Study Design: Developing an <i>in vitro</i> model for assessing the anti-inflammatory properties of curvularin.Purpose: To evaluate the efficacy of natural fungal macrolactone as a therapeutic drug against lipopolysaccharide (LPS)-induced inflammation in primary human nucleus pulposus cells (NPCs) <i>in vitro</i>.Overview of Literature: Lumbar disk disease is a common cause of lower back pain (LBP) and sciatica. It is an established fact that inflammation, rather than mechanical compression on the nerve root, plays a role in the cause of LBP and sciatica. Current treatment options for reducing inflammation are either nonsteroidal anti-inflammatory drugs or steroids, prolonged use of which can potentially lead to adverse effects such as gastrointestinal disturbances and renal and cardiac issues. Hence, there is a need for better antiinflammatory drugs with no or minimal complications for treating inflammation-induced LBP and sciatica. Curvularin (Cur), a fungal macrolactone, is known for its anti-inflammatory activity, but nothing is known about its impact on inflammation due to disk pathologies.Methods: Primary NPCs were cultured and characterized by flow cytometry and immunocytochemistry using the CD24 antibody and treated with 10 μg/mL LPS for 36 hours and then treated with Cur, betamethasone, and dexamethasone (10 μg/mL) for 48 hours, after which cell cycle analysis, cell viability assay, and gene expression studies (quantitative polymerase chain reaction [PCR] and quantitative real-time-PCR) were conducted. The NPCs treated with Cur downregulated the expression of pro-inflammatory cytokines (tumor necrosis factor-α, interleukin [IL]-1β, and IL-6); matrix metalloproteinases (MMPs; MMP-2 and MMP-3), ADAMTS; and apoptotic marker (cytochrome c).Results: In our study, Cur-treated cells showed enhanced expression of collagen 9A1 and insulin-like growth factor receptor 1, indicating the recovery of NPCs from inflammatory assault.Conclusions: Based on observations, the anti-inflammatory properties of Cur render it an excellent drug molecule for treating disk degeneration nonsurgically, by direct injection into spinal disks when treating LBP and sciatica.
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참고문헌 (30)
참고문헌 신청
Banala RR / 2019 / Efficiency of dual siRNA-mediated gene therapy for intervertebral disc degeneration(IVDD) / Spine J 19:896~904
Sampara P / 2018 / Understanding the molecular biology of intervertebral disc degeneration and potential gene therapy strategies for regeneration : a review / Gene Ther 25:67~82
Le Maitre CL / 2007 / Matrix synthesis and degradation in human intervertebral disc degeneration / Biochem Soc Trans 35:652~655
Molinos M / 2015 / Inflammation in intervertebral disc degeneration and regeneration / J R Soc Interface 12:20141191
Devi YS / 2015 / Inhibition of IL-6 signaling pathway by curcumin in uterine decidual cells / PLoS One 10:e0125627
Sampara P / 2018 / Understanding the molecular biology of intervertebral disc degeneration and potential gene therapy strategies for regeneration : a review / Gene Ther 25:67~82
Le Maitre CL / 2007 / Matrix synthesis and degradation in human intervertebral disc degeneration / Biochem Soc Trans 35:652~655
Molinos M / 2015 / Inflammation in intervertebral disc degeneration and regeneration / J R Soc Interface 12:20141191
Devi YS / 2015 / Inhibition of IL-6 signaling pathway by curcumin in uterine decidual cells / PLoS One 10:e0125627
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