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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 253 - 261 (9page)
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BackgroundFluoranthene (FR) is a common environmental pollutant that exists in a complex mixture with other polycyclic aromatic hydrocarbons (PAHs). We identified biomarkers for mon-itoring FR exposure and investigated the rescue effect of FR-induced cellular toxicity via aryl hydrocarbon receptor (AHR) antagonist activity in bone marrow derived mesen-chymal stem cells (BM-MSCs).MethodsMorphological changes, viability, and rescue effects of an AHR antagonist (CH223191) were examined in BM-MSCs after exposure to FR. Cytotoxic effects were assayed using the tetrazolium-based colorimetric assay. Apoptosis was measured by annexin V and pro-pidium iodide dye-based flowcytometry assay, mitochondrial membrane potential assay, and nuclear DNA fragmentation assay. Molecular signaling pathways of apoptosis and autophagy were investigated using immunoblotting. Proteomics were performed in or-der to reveal the spectra of cellular damage and identify biomarkers for FR exposure.ResultsExposing BM-MSCs to FR (IC50=50 M) induced cell death and morphological changes, while the AHR antagonist showed rescue effects. Autophagy was activated and mitochon-drial membrane potential was decreased. Proteomic analysis identified 48 deregulated proteins (26 upregulated and 22 downregulated). Among them, annexin A6, pyruvate kinase, UDP-glucose dehydrogenase, and phospholipase A2 could be potential bio-markers for FR exposure.ConclusionThe exposure of BM-MSCs to FR induced remarkable alterations in cellular biology and the proteome, allowing for identification of novel biomarkers for FR exposure. Furthermore, AHR antagonists might be able to prevent cellular damage due to FR exposure.
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참고문헌 (30)
참고문헌 신청
Raychoudhury SS / 2003 / Polycyclic aromatic hydrocarboninduced cytotoxicity in cultured rat Sertoli cells involves differential apoptotic response / Environ Health Perspect 111:33~38
Cherng SH / 1996 / Modulatory effects of polycyclic aromatic hydrocarbons on the mutagenicity of 1-nitropyrene : a structure-activity relationship study / Mutat Res 367:177~185
Lewtas J / 1997 / Air pollution exposure-DNA adduct dosimetry in humans and rodents : evidence for non-linearity at high doses / Mutat Res 378:51~63
Zedeck MS / 1980 / Polycyclic aromatic hydrocarbons : a review / J Environ Pathol Toxicol Oncol 3:537~567
Busbee DL / 1990 / Comparative uptake, vascular transport, and cellular internalization of aflatoxin-B1 and benzo(a)pyrene / Arch Toxicol 64:285~290
Cherng SH / 1996 / Modulatory effects of polycyclic aromatic hydrocarbons on the mutagenicity of 1-nitropyrene : a structure-activity relationship study / Mutat Res 367:177~185
Lewtas J / 1997 / Air pollution exposure-DNA adduct dosimetry in humans and rodents : evidence for non-linearity at high doses / Mutat Res 378:51~63
Zedeck MS / 1980 / Polycyclic aromatic hydrocarbons : a review / J Environ Pathol Toxicol Oncol 3:537~567
Busbee DL / 1990 / Comparative uptake, vascular transport, and cellular internalization of aflatoxin-B1 and benzo(a)pyrene / Arch Toxicol 64:285~290
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