지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.1
- 수록면
- 957 - 969 (13page)
이용수
초록· 키워드
오류제보하기
Background Brassinosteroids (BRs) are a class of phytohormones with important roles in regulating physiological and developmental processes. Small RNAs, including small interfering RNAs and microRNAs (miRNAs), are non-protein coding RNAs that regulate gene expression at the transcriptional and post-transcriptional levels. However, the roles of small RNAs in BR response have not been studied well. Objective In this study, we aimed to identify BR-responsive small RNA clusters and miRNAs in Arabidopsis. In addition, the effect of BR-responsive small RNAs on their transcripts and target genes were examined. Methods Small RNA libraries were constructed from control and epibrassinolide-treated seedlings expressing wild-type BRI1-Flag protein under its native promoter in the bri1-5 mutant. After sequencing the small RNA libraries, differentially expressed small RNA clusters were identified by examining the expression levels of small RNAs in 100-nt bins of the Arabidopsis genome. To identify the BR-responsive miRNAs, the expression levels of all the annotated mature miRNAs, registered in miRBase, were analyzed. Previously published RNA-seq data were utilized to monitor the BR-responsive expression patterns of differentially expressed small RNA clusters and miRNA target genes. Results In results, 38 BR-responsive small RNA clusters, including 30 down-regulated and eight up-regulated clusters, were identified. These differentially expressed small RNA clusters were from miRNA loci, transposons, protein-coding genes, pseudogenes and others. Of these, a transgene, BRI1, accumulates small RNAs, which are not found in the wild type. Small RNAs in this transgene are up-regulated by BRs while BRI1 mRNA is down-regulated by BRs. By analyzing the expression patterns of mature miRNAs, we have identified BR-repressed miR398a-5p and BR-induced miR156g. Although miR398a5p is down-regulated by BRs, its predicted targets were not responsive to BRs. However, SPL3, a target of BR-inducible miR156g, is down-regulated by BRs. Conclusion BR-responsive small RNAs and miRNAs identified in this study will provide an insight into the role of small RNAs in BR responses in plants. Especially, we suggest that miR156g/SPL3 module might play a role in BR-mediated growth and development in Arabidopsis.
목차
등록된 정보가 없습니다.
참고문헌 (60)
참고문헌 신청
Anders S / 2010 / Differential expression analysis for sequence count data / Genome Biol 11:R106
Baulcombe DC / 2014 / Epigenetic regulation in plant responses to the environment / Cold Spring Harb Perspect Biol 6:a019471
Beauclair L / 2010 / microRNA-directed cleavage and translational repression of the copper chaperone for superoxide dismutase mRNA in Arabidopsis / Plant J 62:454~462
Bologna NG / 2014 / The diversity, biogenesis, and activities of endogenous silencing small RNAs in Arabidopsis / Annu Rev Plant Biol 65:473~503
Bucherl CA / 2013 / Visualization of BRI1 and BAK1(SERK3)membrane receptor heterooligomers during brassinosteroid signaling / Plant Physiol 162:1911~1925
Baulcombe DC / 2014 / Epigenetic regulation in plant responses to the environment / Cold Spring Harb Perspect Biol 6:a019471
Beauclair L / 2010 / microRNA-directed cleavage and translational repression of the copper chaperone for superoxide dismutase mRNA in Arabidopsis / Plant J 62:454~462
Bologna NG / 2014 / The diversity, biogenesis, and activities of endogenous silencing small RNAs in Arabidopsis / Annu Rev Plant Biol 65:473~503
Bucherl CA / 2013 / Visualization of BRI1 and BAK1(SERK3)membrane receptor heterooligomers during brassinosteroid signaling / Plant Physiol 162:1911~1925
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
The complete mitochondrial genome of Arabidopsis thaliana (Brassicaceae) isolated in Korea
식물분류학회지
2021 .06
-
Arabidopsis thaliana as Bioindicator of Fungal VOCs in Indoor Air
Mycobiology
2016 .01
-
Purification, crystallization and X-ray crystallographic analysis of RabA1a from Arabidopsis thaliana
Biodesign
2017 .06
-
Arabidopsis ACC Oxidase 1 Coordinated by Multiple Signals Mediates Ethylene Biosynthesis and Is Involved in Root Development
Molecules and Cells
2018 .10
-
Heterologous Expression and Characterization of a Laccase from Laccaria bicolor in Pichia pastoris and Arabidopsis thaliana
Journal of Microbiology and Biotechnology
2018 .01
-
Effects of Plasma Activated Water on the Root Elongation and Initiation with XTH9, XTH17 Development in Arabidopsis thaliana L.
한국진공학회 학술발표회초록집
2021 .02
-
Conserved co-functional network between maize and Arabidopsis aid in the identification of seed defective genes in maize
Genes & Genomics
2021 .01
-
Extracellular Polymeric Substances of Pseudomonas chlororaphis O6 Induce Systemic Drought Tolerance in Plants
Research in Plant Disease(식물병연구)
2018 .09
-
Arabidopsis WRKY55 Transcription Factor Enhances Soft Rot Disease Resistance with ORA59
The Plant Pathology Journal
2024 .10
-
Investigating the Induced Systemic Resistance Mechanism of 2,4-Diacetylphloroglucinol (DAPG) using DAPG Hydrolase-Transgenic Arabidopsis
The Plant Pathology Journal
2020 .06
-
Suppression of ASK (AtSK32), a Clade III Arabidopsis GSK3, Leads to the Pollen Defect during Late Pollen Development
Molecules and Cells
2015 .06
-
Proteomic Changes in the Sound Vibration-Treated Arabidopsis thaliana Facilitates Defense Response during Botrytis cinerea Infection
The Plant Pathology Journal
2019 .12
-
Profiles of Bacillus spp. Isolated from the Rhizosphere of Suaeda glauca and Their Potential to Promote Plant Growth and Suppress Fungal Phytopathogens
Journal of Microbiology and Biotechnology
2021 .09
-
Optimized Methods for the Isolation of Arabidopsis Female Central Cells and Their Nuclei
Molecules and Cells
2016 .10
-
Humic Acid Confers HIGH-AFFINITY K+ TRANSPORTER 1-Mediated Salinity Stress Tolerance in Arabidopsis
Molecules and Cells
2017 .12
-
Genome-wide analysis of gene expression to distinguish photoperiod-dependent and -independent flowering in Brassicaceae
Genes & Genomics
2017 .01
-
Comparative Analysis of the Conserved Functions of Arabidopsis DRL1 and Yeast KTI12
Molecules and Cells
2015 .03
-
Cell Wall Localization of Two DUF642 Proteins, BIIDXI and TEEBE, during Meloidogyne incognita Early Inoculation
The Plant Pathology Journal
2017 .12
-
An Optimized Method for the Construction of a DNA Methylome from Small Quantities of Tissue or Purified DNA from Arabidopsis Embryo
Molecules and Cells
2021 .08
-
Pyrolysis of Lignin Obtained from Cinnamyl Alcohol Dehydrogenase (CAD) Downregulated Arabidopsis Thaliana
목재공학(Journal of the Korean Wood Science and Technology)
2019 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0