Characterization of transcriptional responses mediated by benzo[a] pyrene stress in a new marine fish model of goby, Mugilogobius chulae

Lei Cai; Jianjun Li; Lujun Yu; Yuanzheng Wei; Zongyu Miao; Meili Chen; Ren Huang

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자료유형: 학술저널

저자정보: Lei Cai (Guangdong Laboratory Animals Monitoring Institute) Jianjun Li (Guangdong Laboratory Animals Monitoring Institute) Lujun Yu (Guangdong Laboratory Animals Monitoring Institute) Yuanzheng Wei (Guangdong Laboratory Animals Monitoring Institute) Zongyu Miao (Guangdong Laboratory Animals Monitoring Institute) Meili Chen (Guangdong Laboratory Animals Monitoring Institute) Ren Huang (Guangdong Laboratory Animals Monitoring Institute)

저널정보: 한국유전학회 Genes & Genomics Genes & Genomics Vol.41 No.1

발행연도: 2019.1

수록면: 113 - 123 (11page)

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연구주제

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연구배경

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연구방법

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Benzo[a]pyrene (BaP) is one of the most studied targets among polycyclic aromatic hydrocarbons (PAHs). Because of the complexity of the toxicity mechanism in BaP, little is known about the molecular mechanism at the level of transcription of BaP in marine fishes. The primary objective of this study was to investigate the molecular basis of the effects of BaP on marine fish, using Mugilogobius chulae (Smith 1932) as the model. A closed colony of M. chulae was used for the BaP toxicity test. Two fish liver samples per replicate from each group were excised and blended into one sample by pooling an equal amount of liver tissue. Total RNA of all samples was extracted separately. Equal quantities of total RNA from the three replicates of the two groups were pooled for sequencing. The sequencing cDNA libraries were sequenced using Illumina HiSeq 2000 system. Differentially expressed genes were detected with the DEGSeq R package. In total, 52,364,032 and 53,771,748 clean nucleotide reads were obtained in the control and BaP-exposed libraries, respectively, with N50 lengths of 1277 and 1288 bp, respectively. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses revealed a significant enrichment of genes related to detoxification, transportation, and lipid metabolism. We also identified, for the first time, an association between endoplasmic reticulum dysfunction and lipid metabolism resulting from BaP exposure. Using quantitative real-time PCR, some effective molecular biomarkers for monitoring of BaP-polluted seawater were identified. The results demonstrate that BaP enhanced the expression of genes involved in detoxification in M. chulae and inhibited that of genes related to lipid metabolism, possibly by suppressing the expression of numerous ER-related genes involved in fat digestion and absorption.

#Transcriptome sequencing · Mugilogobius chulae · Benzo[a]pyrene · Xenobiotic metabolism · Lipid metabolism · Endoplasmic reticulum

참고문헌 (67)

참고문헌 신청

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