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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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Brucella abortus is an intracellular bacterial pathogen that causes brucellosis in humans and livestock. The genome of B. abortus encodes the VirB type IV secretion system (T4SS), which is essential to its virulence. B. abortus produces and secretes effector proteins through the T4SS to survive in the intracellular environment and manipulate host immunity. The T4SS spans the peptidoglycan layer through vacancies in the peptidoglycan chain. Recently, secretion activator gene A (SagA) from B. abortus was identified as a lysozyme-like enzyme that creates holes in the peptidoglycan layer. In this study, SagA from B. abortus was overexpressed, purified, and crystallized. Crystal diffraction data were acquired at 2.0 A resolution, a P213 space group with a unit cell parameter of 79.04 A. We are currently exploring the crystal structure of SagA using the anomalous signal from selenomethionine-substituted crystals and an X-ray free-electron laser.
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참고문헌 (14)
참고문헌 신청
Abdallah, A. M. / 2007 / Type VII secretion--mycobacteria show the way / Nat Rev Microbiol 5:883~891
Archambaud, C. / 2010 / Contrasting roles of macrophages and dendritic cells in controlling initial pulmonary Brucella infection / Eur J Immunol 40:3458~3471
Comerci, D. J. / 2001 / Essential role of the VirB machinery in the maturation of the Brucella abortus-containing vacuole / Cell Microbiol 3:159~168
Del Giudice, M. G. / 2019 / PhiA, a peptidoglycan hydrolase inhibitor of Brucella involved in the virulence process / Infect Immun 87:e00352~19
Del Giudice, M. G. / 2013 / A lysozyme-like protein in Brucella abortus is involved in the early stages of intracellular replication / Infect Immun 81:956~964
Archambaud, C. / 2010 / Contrasting roles of macrophages and dendritic cells in controlling initial pulmonary Brucella infection / Eur J Immunol 40:3458~3471
Comerci, D. J. / 2001 / Essential role of the VirB machinery in the maturation of the Brucella abortus-containing vacuole / Cell Microbiol 3:159~168
Del Giudice, M. G. / 2019 / PhiA, a peptidoglycan hydrolase inhibitor of Brucella involved in the virulence process / Infect Immun 87:e00352~19
Del Giudice, M. G. / 2013 / A lysozyme-like protein in Brucella abortus is involved in the early stages of intracellular replication / Infect Immun 81:956~964
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Structure and Function of the Autolysin SagA in the Type IV Secretion System of Brucella abortus
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Substantial Protective Immunity Conferred by a Combination of Brucella abortus Recombinant Proteins against Brucella abortus 544 Infection in BALB/c Mice
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β-Sitosterol Contributes in the Resistance to Invasion and Survival of Brucella abortus 544 within RAW264.7 Cells, and Cytokine Production with Reduced Susceptibility to Infection in BALB/c Mice
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The Bactericidal Effect of High Temperature Is an Essential Resistance Mechanism of Chicken Macrophage against Brucella abortus Infection
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Genes Related to Intracellular Survival of Brucella abortus in THP-1 Macrophage Cells
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Immunization of Mice with Recombinant Brucella abortus Organic Hydroperoxide Resistance (Ohr) Protein Protects Against a Virulent Brucella abortus 544 Infection
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Intracellular Trafficking Modulation by Ginsenoside Rg3 Inhibits Brucella abortus Uptake and Intracellular Survival within RAW 264.7 Cells
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Modulatory Effect of Linoleic Acid During Brucella abortus 544 Infection in Murine Macrophage RAW264.7 Cells and Murine Model BALB/c Mice
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Inhibitory Effect of the Ethanol Extract of a Rice Bran Mixture Comprising Angelica gigas, Cnidium officinale, Artemisia princeps, and Camellia sinensis on Brucella abortus Uptake by Professional and Nonprofessional Phagocytes
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Immune Modulation of Recombinant OmpA against Brucella abortus 544 Infection in Mice
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Protective Effects against Brucella abortus 544 Infection in a Murine Macrophage Cell Line and in a Mouse Model via Treatment with Sirtuin 1 Activators Resveratrol, Piceatannol and Ginsenoside Rg3
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The In Vitro and In Vivo Effect of Lipoxygenase Pathway Inhibitors Nordihydroguaiaretic Acid and Its Derivative Tetra- O -methyl Nordihydroguaiaretic Acid against Brucella abortus 544
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Emodin Successfully Inhibited Invasion of Brucella abortus Via Modulting Adherence, Microtubule Dynamics and ERK Signaling Pathway in RAW 264.7 Cells
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Evaluation of Th1/Th2-Related Immune Response against Recombinant Proteins of Brucella abortus Infection in Mice
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Type three secretion system in Salmonella Typhimurium: the key to infection
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The Peptidoglycan as a Signaling Molecule of Bacterial Origin Shaping Host Physiology
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SAGA Coordinates Stress-induced mRNA Quality Control and Export for Proteostasis
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Effect of Peptidoglycan Carboxypeptidases on Antibiotic Resistance
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Native and Foreign Proteins Secreted by the Cupriavidus metallidurans Type II System and an Alternative Mechanism
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