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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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Background: Cardio myoblast generation from conventional approaches is laborious and time-consuming. We present a bioelectronics on-a-chip for stimulating cells cardio myoblast proliferation during culture. Method: The bioelectronics chip fabrication methodology involves two different process. In the first step, an aluminum layer of 200 nm is deposited over a soda-lime glass substrate using physical vapor deposition and selectively removed using a Q-switched Nd:YVO4 laser to create the electric tracks. To perform the experiments, we developed a biochip composed of a cell culture chamber fabricated with polydimethylsiloxane (PDMS) with a glass coverslip or a cell culture dish placed over the electric circuit tracks. By using such a glass cover slip or cell culture dish we avoid any toxic reactions caused by electrodes in the culture or may be degraded by electrochemical reactions with the cell medium, which is crucial to determine the effective cell-device coupling. Results: The chip was used to study the effect of electric field stimulation of Rat ventricular cardiomyoblasts cells (H9c2). Results shows a remarkable increase in the number of H9c2 cells for the stimulated samples, where after 72 h the cell density double the cell density of control samples. Conclusions: Cell proliferation of Rat ventricular cardiomyoblasts cells (H9c2) using the bioelectronics-on-a-chip was enhanced upon the electrical stimulation. The dependence on the geometrical characteristics of the electric circuit on the peak value and homogeneity of the electric field generated are analyzed and proper parameters to ensure a homogeneous electric field at the cell culture chamber are obtained. It can also be observed a high dependence of the electric field on the geometry of the electrostimulator circuit tracks and envisage the potential applications on electrophysiology studies, monitoring and modulate cellular behavior through the application of electric fields.
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참고문헌 (41)
참고문헌 신청
Emmert MY / 2014 / Cell therapy, 3-D culture systems and tissue engineering for cardiac regeneration / Adv Drug Deliv Rev 69-70:254~269
Banks, T.A. / 2015 / Effects of electric fields on human mesenchymal stem cell behavior and morphology using a novel multichannel device / Integr Biol 7:693~712
Pavesi A / 2014 / Electrical conditioning of adiposederived stem cells in a multi-chamber culture platform / Biotechnol Bioeng 111(7):1452~1463
Serena E / 2009 / Electrical stimulation of human embryonic stem cells : cardiac differentiation and the generation of reactive oxygen species / Exp Cell Res 315(20):3611~3619
Dehail P / 2008 / Electrical stimulation and muscle strengthening / Ann Readapt Med Phys 51:441~451
Banks, T.A. / 2015 / Effects of electric fields on human mesenchymal stem cell behavior and morphology using a novel multichannel device / Integr Biol 7:693~712
Pavesi A / 2014 / Electrical conditioning of adiposederived stem cells in a multi-chamber culture platform / Biotechnol Bioeng 111(7):1452~1463
Serena E / 2009 / Electrical stimulation of human embryonic stem cells : cardiac differentiation and the generation of reactive oxygen species / Exp Cell Res 315(20):3611~3619
Dehail P / 2008 / Electrical stimulation and muscle strengthening / Ann Readapt Med Phys 51:441~451
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