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논문 기본 정보
- 자료유형
- 학술대회자료
- 저자정보
- 발행연도
- 2021.7
- 수록면
- 192 - 192 (1page)
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The adenocarcinomic human alveolar basal epithelial cells (A549) are commonly used for in vitro cell models of respiratory toxicity. In this study, we intended to establish in vitro culture models for respiratory toxicity testing by comparing air-liquid interface (ALI) conditions of A549 cells with liquid-liquid interface (LLI). A549 cells were seeded at 1*10^5 cells/well in an apical compartment of 12 well insert (Greiner Bio-one). Culture medium was added to the basolateral compartment and was replaced every 2-3 days in contact with cells for nutrient supply via the membrane. The medium was added only to the basolateral compartment in ALI culture condition while it was added to bot ... 전체 초록 보기
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