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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.1
- 수록면
- 766 - 777 (12page)
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초록· 키워드
오류제보하기
Bezafibrate, a fibrate drug used as a lipid-lowering agent to treat hyperlipidemia, is a pan-agonist of peroxisome proliferator-activated receptor alpha. It can enhance mitochondrial fatty acid oxidation, oxidative phosphorylation, and mitochondrial biogenesis. After ovulation, oocytes may get arrested at the metaphase II (MII) stage until fertilization beyond optimal timing, which is termed as post-ovulatory aging. Post-ovulatory aging is a disease that degrades DNA, mitochondria, and oxidative system, and has a negative impact on embryo development and quality; however, the impact of bezafibrate during post-ovulatory aging has not been fully defined. In the present study, we assessed the ability of bezafibrate to prevent the progression of aging in in vitro conditions as well as the underlying mechanisms in pigs. An appropriate concentration of this drug (50 μM) was added, and then oxidative stress, reactive oxygen species downstream, mitochondrial biogenesis, and mitochondrial function were analyzed via immunofluorescence staining and real-time polymerase chain reaction. Bezafibrate significantly alleviated reactive oxygen species and ameliorated glutathione production simultaneously in oocytes and embryos. Moreover, it diminished H2A.X and attenuated CASPASE 3 expression produced by oxidative stress in oocytes and embryos. Furthermore, bezafibrate remarkably improved the mitochondrial function and blastocyst quality as well as markedly reduced the mitochondria/TOM20 ratio and mtDNA copy number. The elevated PARKIN level indicated that mitophagy was induced by bezafibrate treatment after post-ovulatory aging. Collectively, these results suggest that bezafibrate beneficially affects against porcine post-ovulatory oocyte aging in porcine by its antioxidant property and mitochondrial protection.
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등록된 정보가 없습니다.
참고문헌 (25)
참고문헌 신청
Qing-Yuan Sun / 2009 / Towards a new understanding on the regulation of mammalian oocyte meiosis resumption / Cell Cycle 8(17):2741~2747
Tessa Lord / 2013 / Oxidative stress and ageing of the post-ovulatory oocyte / REPRODUCTION 146(6):R217~R227
A. L. Mikkelsen / 2001 / Morphology of in-vitro matured oocytes: impact on fertility potential and embryo quality / Human Reproduction 16(8):1714~1718
Maggie M.-Y. Chi / 1988 / Contrast in Levels of Metabolic Enzymes in Human and Mouse Ova1 / Biology of Reproduction 39(2):295~307
Toshifumi Takahashi / 2003 / Impact of oxidative stress in aged mouse oocytes on calcium oscillations at fertilization / Molecular Reproduction and Development 66(2):143~152
Tessa Lord / 2013 / Oxidative stress and ageing of the post-ovulatory oocyte / REPRODUCTION 146(6):R217~R227
A. L. Mikkelsen / 2001 / Morphology of in-vitro matured oocytes: impact on fertility potential and embryo quality / Human Reproduction 16(8):1714~1718
Maggie M.-Y. Chi / 1988 / Contrast in Levels of Metabolic Enzymes in Human and Mouse Ova1 / Biology of Reproduction 39(2):295~307
Toshifumi Takahashi / 2003 / Impact of oxidative stress in aged mouse oocytes on calcium oscillations at fertilization / Molecular Reproduction and Development 66(2):143~152
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