지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 충남대학교 농업과학연구소 Korean Journal of Agricultural Science Korean Journal of Agricultural Science Vol.48 No.1
- 발행연도
- 2021.3
- 수록면
- 163 - 170 (8page)
이용수
초록· 키워드
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Bacterial infections in the female reproductive tract negatively affect ovarian function, follicular development, and embryo development, leading to the eventual failure of fertilization. Moreover, bacterial lipopolysaccharides (LPS) can interfere with the immune system and reproductive system of the host animal. Therefore, this study examined the effect of LPS on the in vitro maturation (IVM) of pig oocytes. Oocytes were matured in TCM199 medium in the presence of varying concentrations of LPS (0 - 50 μg·mL<SUP>-1</SUP>). The maturation rate, cortical granules (CGs) migration, and chromosome alignment were subsequently evaluated during the meiotic development of the oocytes. We observed a dose-dependent and significant decrease in the metaphase II (MII) rate with increasing concentrations of LPS (97.6% control [0 μg·mL<SUP>-1</SUP> LPS] vs. 10.4-74.9% LPS [1 - 50 μg·mL<SUP>-1</SUP>], p < 0.05). In addition, compared to the control oocytes without LPS, higher levels of abnormal CGs distribution (18.1 - 50.0% LPS vs. 0% control), chromosome/spindle alignment (20.3 - 56.7% LPS vs. 0% control), and intracellular ROS generation were observed in oocytes matured with LPS (p < 0.05). Nitrite levels were also increased in the maturation medium derived from the oocytes matured with LPS (p < 0.05). These results indicate that LPS induces oxidative stress during IVM and affects oocyte maturation, including CGs migration and chromosome alignment of pig oocytes.
목차
Abstract
Introduction
Materials and Methods
Results and Discussion
Conclusion
References
참고문헌 (31)
참고문헌 신청
Abeydeera LR / 1998 / Maturation in vitro of pig oocytes in protein-free culture media : Fertilization and subsequent embryo development in vitro / Biology of Reproduction 58:1316~1320
Angrisano T / 2010 / LPS-induced IL-8 activation in human intestinal epithelial cells is accompanied by specific histone H3 acetylation and methylation changes / BMC microbiology 10:1~8
Borges ED / 2020 / Microbial contamination in assisted reproductive technology : Source, prevalence, and cost / Journal of Assisted Reproduction and Genetics 37:53~61
Bromfield JJ / 2011 / Lipopolysaccharide initiates inflammation in bovine granulosa cells via the TLR4pathway and perturbs oocyte meiotic progression in vitro / Endocrinology 152:5029~5040
Coleman JW / 2001 / Nitric oxide in immunity and inflammation / International Immunopharmacology 1:1397~1406
Angrisano T / 2010 / LPS-induced IL-8 activation in human intestinal epithelial cells is accompanied by specific histone H3 acetylation and methylation changes / BMC microbiology 10:1~8
Borges ED / 2020 / Microbial contamination in assisted reproductive technology : Source, prevalence, and cost / Journal of Assisted Reproduction and Genetics 37:53~61
Bromfield JJ / 2011 / Lipopolysaccharide initiates inflammation in bovine granulosa cells via the TLR4pathway and perturbs oocyte meiotic progression in vitro / Endocrinology 152:5029~5040
Coleman JW / 2001 / Nitric oxide in immunity and inflammation / International Immunopharmacology 1:1397~1406
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UCI(KEPA) : I410-ECN-0101-2021-520-001687702