지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.1
- 수록면
- 272 - 280 (9page)
이용수
초록· 키워드
오류제보하기
Cumulus-oocyte complexes (COCs), which contain immature oocytes, are matured in vitro for in vitro embryo production. Oocyte and cumulus cells are then separated using hyaluronidase. To date, there have only been a few reported cases of the toxic effects of hyaluronidase on porcine oocytes. The aim of this study was to compare the effects of bovine testis-derived hyaluronidase and recombinant human hyaluronidase on oocyte denudation and quality. Porcine COCs were matured for 44 h and denuded using different hyaluronidase concentrations and exposure times. Then, oocytes were activated by electrical parthenogenesis. In experiment 1, COCs were denuded using bovine-derived, ovine-derived (Hirax), and human recombinant (ALT-BC4) hyaluronidases for 10 and 20 min. In experiment 2, bovine-derived and human recombinant (ALT-BC4 and ICSI Cumulase®) hyaluronidases were used to denude the COCs for 2 and 20 min. In both experiments the oocytes were all completely denuded, and there was no degeneration. Rate of embryo development was significantly increased in group treated ALT-BC4 for 2 min and not significantly different in other treatment groups. In general it slightly decreased with longer exposure times. These results have confirmed that different sources of hyaluronidase do not have detrimental effects on the quality of porcine oocytes and suggest that the human recombinant hyaluronidase ALT-BC4 is suitable for oocyte denudation with an increased blastocyst rate.
목차
등록된 정보가 없습니다.
참고문헌 (15)
참고문헌 신청
R. Stern / 2003 / Devising a pathway for hyaluronan catabolism: are we there yet? / Glycobiology 13(12):105R~115
Antonella Camaioni / 1996 / Proteoglycans and Proteins in the Extracellular Matrix of Mouse Cumulus Cell–Oocyte Complexes / Archives of Biochemistry and Biophysics 325(2):190~198
Abdul-Aziz M / 1995 / Hyaluronidase activity in human semen : correlation with fertilization in vitro / Fertil steril 64:1147~1153
Tyl H. Taylor / 2006 / Comparison of bovine- and recombinant human-derived hyaluronidase with regard to fertilization rates and embryo morphology in a sibling oocyte model: a prospective, blinded, randomized study / Fertility and Sterility 85(5):1544~1546
Melanie Evison / 2009 / Human recombinant hyaluronidase (Cumulase®) improves intracytoplasmic sperm injection survival and fertilization rates / Reproductive BioMedicine Online 18(6):811~814
Antonella Camaioni / 1996 / Proteoglycans and Proteins in the Extracellular Matrix of Mouse Cumulus Cell–Oocyte Complexes / Archives of Biochemistry and Biophysics 325(2):190~198
Abdul-Aziz M / 1995 / Hyaluronidase activity in human semen : correlation with fertilization in vitro / Fertil steril 64:1147~1153
Tyl H. Taylor / 2006 / Comparison of bovine- and recombinant human-derived hyaluronidase with regard to fertilization rates and embryo morphology in a sibling oocyte model: a prospective, blinded, randomized study / Fertility and Sterility 85(5):1544~1546
Melanie Evison / 2009 / Human recombinant hyaluronidase (Cumulase®) improves intracytoplasmic sperm injection survival and fertilization rates / Reproductive BioMedicine Online 18(6):811~814
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Rapamycin treatment during prolonged in vitro maturation enhances the developmental competence of immature porcine oocytes
한국축산학회지
2024 .09
-
DNA damage repair is suppressed in porcine aged oocytes
한국축산학회지
2021 .09
-
Localization of Autophagosome in Porcine Follicular Cumulus-oocyte Complex
한국수정란이식학회지
2017 .01
-
소 난자의 체외성숙시 난구세포와 난세포질에서 다르게 발현되는 Matrix Metalloproteinases의 분석
한국수정란이식학회지
2018 .01
-
Adverse effect of IL-6 on the in vitro maturation of porcine oocytes
Korean Journal of Agricultural Science
2021 .09
-
Influence of Autophagy Induction after Hormone Treatment on Oocytes Maturation of Porcine
한국수정란이식학회지
2018 .01
-
Effect of Alpha Lipoic Acid on in vitro Maturation of Porcine Oocytes and Subsequent Embryonic Development after Parthenogenetic Activation
한국수정란이식학회지
2017 .01
-
Antioxidant Effect of Alpha-Linolenic Acid during In Vitro Maturation in Porcine Oocytes
한국동물번식학회지
2017 .01
-
The Beneficial Effects of Ferulic Acid supplementation during In Vitro Maturation of Porcine Oocytes on Their Parthenogenetic Development
한국수정란이식학회지
2017 .01
-
Screening and compound isolation from selected Pakistan medicine plants for anti-hyaluronidase and anti-oxidant activities
한국식품영양과학회 학술대회발표집
2023 .10
-
Heat waves impair cytoplasmic maturation of oocytes and preimplantation development in Korean native cattle (Hanwoo)
Korean Journal of Agricultural Science
2018 .09
-
Effect of Monosodium Glutamate on In Vitro Oocyte Maturation and Embryonic Development after Parthenogenesis in Pigs
한국수정란이식학회지
2017 .01
-
Effects of (-)-Epicatechin Gallate on porcine oocyte in vitro maturation and subsequent embryonic development after parthenogenetic activation and in vitro fertilization
한국수정란이식학회지
2016 .01
-
Changes of Ganglioside GM3 Expression in Porcine Oocyte Maturation and Early Embryonic Development In Vitro
한국수정란이식학회지
2015 .01
-
Effects of lipopolysaccharides on the maturation of pig oocytes
Korean Journal of Agricultural Science
2021 .03
-
Endoplasmic Stress Inhibition during Oocyte Maturation Improves Preimplantation Development of Cloned Pig Embryos
한국수정란이식학회지
2017 .01
-
돼지 난자의 체외성숙에서 합성배양액에 첨가된 과당이 난자의 성숙 및 단위발생 배아의 체외발육에 미치는 영향
한국수정란이식학회지
2017 .01
-
Treatment of Exogenous GDF9 and BMP15 during In Vitro Maturation of Oocytes increases the Cell Number of Blastocysts in Pigs
한국수정란이식학회지
2016 .01
-
Heat Stress가 소 난자의 체외성숙과 배반포 발달에 미치는 영향
한국수정란이식학회지
2017 .01
-
The influence and role of melatonin on in vitro oocyte maturation and embryonic development in pig and cattle
Korean Journal of Agricultural Science
2017 .09
이 논문의 저자 정보
최근 본 자료
댓글(0)
0