지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
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The detection of Mycobacterium bovis (M. bovis) in environmental samples with precision is imperative to control bovine tuberculosis (bTB) infections at the herd level, as residual M. bovis remains one of the major causes of recurring infections. In this study, a nested PCR method for the detection of M. bovis in environmental samples was applied to identify potential environmental reservoirs of the bacterium. A set of 200 environmental samples (167 fecal samples and 33 water samples) from 39 herds with a history of bTB outbreak was analyzed using a nested PCR method to detect residual M. bovis. Amplicon libraries of the IS6110 target gene fragment were amplified from M. bovis DNA using two established primer sets. A positive nested PCR result was observed in 69.5% of fecal samples and 66.7% of water samples, thus showing that residual M. bovis was present in the environmental samples of bTB-positive herds in a high proportion. This study is the first to demonstrate high levels of M. bovis DNA in environmental samples and to show that environmental reservoirs of this pathogen contribute to recurring outbreaks of bTB. Environmental monitoring of herds in which bTB outbreaks have occurred with high sensitivity and specificity is expected to help prevent the recurrence of potential bTB disease and improve the herd environment.
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참고문헌 (29)
참고문헌 신청
Adams AP / 2013 / Comparison of PCR versus culture for detection of Mycobacterium bovis after experimental inoculation of various matrices held under environmental conditions for extended periods / Appl Environ Microbiol 79(20):6501~6506
Aranaz A / 2004 / Bovine tuberculosis(Mycobacterium bovis)in wildlife in Spain / J Clin Microbiol 42(6):2602~2608
Araujo CP / 2014 / Detection of Mycobacterium bovis in bovine and bubaline tissues using nested-PCR for TbD1 / PLoS One 9(3):e91023
De Groote MA / 2006 / Relationships between Mycobacterium isolates from patients with pulmonary mycobacterial infection and potting soils / Appl Environ Microbiol 72(12):7602~7606
Duffield BJ / 1985 / Survival of Mycobacterium bovis in defined environmental conditions / Vet Microbiol 10(2):193~197
Aranaz A / 2004 / Bovine tuberculosis(Mycobacterium bovis)in wildlife in Spain / J Clin Microbiol 42(6):2602~2608
Araujo CP / 2014 / Detection of Mycobacterium bovis in bovine and bubaline tissues using nested-PCR for TbD1 / PLoS One 9(3):e91023
De Groote MA / 2006 / Relationships between Mycobacterium isolates from patients with pulmonary mycobacterial infection and potting soils / Appl Environ Microbiol 72(12):7602~7606
Duffield BJ / 1985 / Survival of Mycobacterium bovis in defined environmental conditions / Vet Microbiol 10(2):193~197
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