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Forensic Microbiological Analysis for Vaginal Fluid Identification Using Multiplex Real-Time PCR System
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실시간 PCR System을 이용한 법미생물학적 질액 식별

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Type
Academic journal
Author
Jeongyong Kim (국립과학수사연구원 법유전자과)
Journal
대한법의학회 대한법의학회지 대한법의학회지 제45권 제1호 KCI Accredited Journals
Published
2021.1
Pages
14 - 21 (8page)

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Forensic Microbiological Analysis for Vaginal Fluid Identification Using Multiplex Real-Time PCR System
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Numerous methods for human body fluid identification using microbiological markers specific to different human body parts are well-established in forensic science. However, method for vaginal fluid screening have not been standardized yet. Therefore, in this study, a real-time polymerase chain reaction based assay for vaginal fluid identification was devised using bacteria residing in human vagina. This method employed three markers, namely Lactobacillus iners, Lactobacillus crispatus, and Bacteroides fragilis. L. iners and L. crispatus were chosen due to their high abundance in the vagina, whereas B. fragilis resides in the rectum. To examine the suitability of the new method for forensic microbial applications, a study of the distribution of vaginal flora in 143 Korean women was performed, along with characterization of the specificity, and performance of the new assay. Additionally, a casework study based on 130, 21, 20 and 17 DNA samples collected from the vagina, anus, saliva, blood, respectively, was carried out. L. iners (80.4%) and L. crispatus (55.2%) were detected with high abundance in the vagina of Korean women. The specificity of these markers was verified using microbial DNA from 23 species. This method could detect at least 1,000 copies/μL of microorganisms for all markers, thereby highlighting its robust sensitivity for vaginal fluid identification. The casework study confirmed these findings, with 89.2% (116/130) detection of vaginal fluid-derived DNA samples, and no false positives identified from the other sources studied. In conclusion, the developed method is expected to be efficient for preliminary microbiological analysis of vaginal samples in forensics.
#Real-time polymerase chain reaction #Microbiology #Bacterial genome #Vaginal bacteria #Vaginal fluid identification

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References (32)

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Costello EK / 2009 / Bacterial community variation in human body habitats across space and time / Science 326:1694~1697 google schola Lehman DC / 2012 / Forensic microbiology / Clin Lab Sci 25:114~119 google schola Pang BC / 2008 / Applicability of two commercially available kits for forensic identification of saliva stains / J Forensic Sci 53:1117~1122 google schola Juusola J / 2003 / Messenger RNA profiling: a prototype method to supplant conventional methods for body fluid identification / Forensic Sci Int 135:85~96 google schola Ja Hyun An / 2012 / Body fluid identification in forensics / BMB Reports 45(10):545~553 dbpia

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