Initiation, proliferation, and improvement of a micropropagation system  for mass clonal production of banana through shoot-tip culture

Allah Jurio Khaskheli; Muharam Ali; Syad Zakir Hussain Shah; Zohra Fatima Memon; Saleem Awan; Muhammad Ibrahim Khaskheli; Mohsin Ali Khaskheli; Bilqees Magsi; Zareen Qambrani; Asad Ali Khaskheli

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자료유형: 학술저널

저자정보: Allah Jurio Khaskheli (Sindh Agriculture University) Muharam Ali (Sindh Agriculture University) Syad Zakir Hussain Shah (Sindh Agriculture University) Zohra Fatima Memon (Sindh Agriculture University) Saleem Awan (Sindh Agriculture University) Muhammad Ibrahim Khaskheli (Sindh Agriculture University) Mohsin Ali Khaskheli (Sindh Agriculture University) Bilqees Magsi (Sindh Agriculture University) Zareen Qambrani (Sindh Agriculture University) Asad Ali Khaskheli (Sindh Agriculture University)

저널정보: 한국식물생명공학회 Journal of Plant Biotechnology Journal of Plant Biotechnology 제48권 제2호

발행연도: 2021.1

수록면: 86 - 92 (7page)

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The present study aimed to evaluate the initiation, proliferation potential, and mass clonal production ability of a micropropagation system for banana through tissue culture. A total of 60 explants were cultured on basal media supplemented with various concentrations of BAP and NAA. Banana plants regenerated on MS basal medium (control) without the addition of BAP + NAA showed a significantly (P < 0.05) lower survival rate with no signs of shoots up to the end of the experimental period. The results further revealed that the performance in MSS-XI medium was almost 89%, followed by MSS-IX and MSS-X media, both of which showed performance up to 88%. In contrast, the performance in the MSS-XVI medium was less than 60%, at the less duration of time and highly shoot induction detected at MSS-XIII medium. The maximum number of shoots (4.9) was observed in the medium supplemented with growth adjuster MSS-XI, followed by the MSS-XII medium (4.5). Surprisingly, the best performance was observed for the MSR-VII medium approximately 16 days after initiation, while the lowest performance was observed with MSR-XI (approximately 31 days). The maximum rooting percentage (98%) was observed in the MSR-V to MSR-VIII media (98%), while the minimum rooting percentage was observed in MSR-XI (approximately 45%)

#Banana #Tissue culture #Hormones #Regeneration #Shoots #Roots

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