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논문 기본 정보

자료유형
학술저널
저자정보
Waghmare Pankajkumar Ramdas (State Key Laboratory of Microbial Technology Shandong University Shandong 266237 P. R. China) Waghmare Pratima Pankajkumar (State Key Laboratory of Microbial Technology Shandong University Shandong 266237 P. R. China) Liwei Gao (State Key Laboratory of Microbial Technology Shandong University Shandong 266237 P. R. ChinaTobacco) Sun Wan (State Key Laboratory of Microbial Technology Shandong University Shandong 266237 P. R. ChinaNationa) Qin Yuqi (State Key Laboratory of Microbial Technology Shandong University Shandong 266237 P. R. ChinaNationa) Guodong Liu (State Key Laboratory of Microbial Technology Shandong University Shandong 266237 P. R. ChinaNationa) Qu Yinbo (State Key Laboratory of Microbial Technology Shandong University Shandong 266237 P. R. ChinaNationa)
저널정보
한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제31권 제5호
발행연도
2021.1
수록면
740 - 746 (7page)

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Efficient cellulolytic enzyme production is important for the development of lignocellulosedegrading enzyme mixtures. However, purification of cellulases from their native hosts is time- and labor-consuming. In this study, a constitutive expression system was developed in Penicillium oxalicum for the secreted production of proteins. Using a constitutive polyubiquitin gene promoter and cultivating with glucose as the sole carbon source, nine cellulolytic enzymes of different origins with relatively high purity were produced within 48 h. When supplemented to a commercial cellulase preparation, cellobiohydrolase I from P. funiculosum and cellobiohydrolase II from Talaromyces verruculosus showed remarkable enhancing effects on the hydrolysis of steam-exploded corn stover. Additionally, a synergistic effect was observed for these two cellobiohydrolases during the hydrolysis. Taken together, the constitutive expression system provides a convenient tool for the production of cellulolytic enzymes, which is expected to be useful in the development of highly efficient lignocellulose-degrading enzyme mixtures.

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