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논문 기본 정보

자료유형
학술저널
저자정보
Liu Yang (State Key Laboratory of Food Science and Technology Jiangnan University Wuxi Jiangsu 214122 P. R. C) Li Yifeng (State Key Laboratory of Food Science and Technology Jiangnan University Wuxi Jiangsu 214122 P. R. C) Yu Xinjie (Hwa Chong Institution (College) 661 Bukit Timah Road Singapore 269734 Singapore) Yu Leilei (State Key Laboratory of Food Science and Technology Jiangnan University Wuxi Jiangsu 214122 P. R. C) Tian Fengwei (State Key Laboratory of Food Science and Technology Jiangnan University Wuxi Jiangsu 214122 P. R. C) Zhao Jianxin (State Key Laboratory of Food Science and Technology Jiangnan University Wuxi Jiangsu 214122 P. R. C) Zhang Hao (State Key Laboratory of Food Science and Technology China) Zhai Qixiao (State Key Laboratory of Food Science and Technology Jiangnan University Wuxi Jiangsu 214122 P. R. C) Chen Wei (Jiangnan University China)
저널정보
한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제31권 제1호
발행연도
2021.1
수록면
92 - 103 (12page)

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Lactobacillus casei, one of the most widely used probiotics, has been reported to alleviate multiple diseases. However, the effects of this species on intestinal diseases are strain-specific. Here, we aimed to screen L. casei strains with inflammatory bowel disease (IBD)-alleviating effects based on in vitro physiological characteristics. Therefore, the physiological characteristics of 29 L. casei strains were determined, including gastrointestinal transit tolerance, oligosaccharide fermentation, HT-29 cell adhesion, generation time, exopolysaccharide production, acetic acid production, and conjugated linoleic acid synthesis. The effects of five candidate strains on mice with induced colitis were also evaluated. The results showed that among all tested L. casei strains, only Lactobacillus casei M2S01 effectively relieved colitis. This strain recovered body weight, restored disease activity index score, and promoted anti-inflammatory cytokine expression. Gut microbiota sequencing showed that L. casei M2S01 restored a healthy gut microbiome composition. The western blotting showed that the alleviating effects of L. casei M2S01 on IBD were related to the inhibition of the NF- κB pathway. A good gastrointestinal tolerance ability may be one of the prerequisites for the IBDalleviating effects of L. casei. Our results verified the efficacy of L. casei in alleviating IBD and lay the foundation for the rapid screening of L. casei strain with IBD-alleviating effects.

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