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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제30권 제10호
- 발행연도
- 2020.1
- 수록면
- 1,536 - 1,542 (7page)
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Dekkera anomala YAE-1 strain separated from “airag” (Mongolian fermented mare’s milk) produces β-glucosidase, which can convert ginsenoside Rb1 from Panax ginseng. Ginseng- derived bioactive components such as ginsenoside Rb1 have various immunological and anticancer activities. Airag was collected from five different mare milk farms located near Ulaanbaatar, Mongolia. YAE-1 strains were isolated from airag to examine the hydrolytic activities of β-glucosidase on Korean Panax ginseng using an API ZYM kit. Supernatants of selected cultures having β-glucosidase activity were examined for hydrolysis of the major ginsenoside Rb1 at 40°C, pH 5.0. The YAE-1 strain was found to be nearly identical at 99.9% homology with Dekkera anomala DB-7B, and was thus named Dekkera anomala YAE-1. This strain exerted higher β?glucosidase activity than other enzymes. Reaction mixtures from Dekkera anomala YAE-1 showed great capacity for converting ginsenoside Rb1 to ginsenoside Rd. The β-glucosidase produced by Dekkera anomala YAE-1 was able to hydrolyze ginsenoside Rb1 and convert it to Rd during fermentation of the ginseng. The amount of ginsenoside Rd was highly increased from 0 to 1.404 mg/ml in fermented 20% ginseng root at 7 days.
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참고문헌 (22)
참고문헌 신청
imFactbook / imFact Publishing Co. Korea
Kitagawa I / 1987 / Chemical studies on crude drug processing. V. on the constituents of ginseng radix rubra(2) : Comparison of the constituents of white ginseng and red ginseng prepared from the same Panax ginseng root / Yakugaku Zasshi 107:495~505
Kim M / 1987 / Distribution of saponin in various sections of Panax ginseng root and changes of its contents according to root age / Korean J. Ginseng Sci 11:10~16
Son JW / 2008 / Ginsenoside Rd production from the major ginsenoside Rb(1)by beta-glucosidase from Thermus caldophilus / Biotechnol. Lett 30:713~716
Wang L / 2012 / Immunosuppressive effects of ginsenoside-Rd on skin allograft rejection in rats / J. Surg. Res 176:267~274
Kitagawa I / 1987 / Chemical studies on crude drug processing. V. on the constituents of ginseng radix rubra(2) : Comparison of the constituents of white ginseng and red ginseng prepared from the same Panax ginseng root / Yakugaku Zasshi 107:495~505
Kim M / 1987 / Distribution of saponin in various sections of Panax ginseng root and changes of its contents according to root age / Korean J. Ginseng Sci 11:10~16
Son JW / 2008 / Ginsenoside Rd production from the major ginsenoside Rb(1)by beta-glucosidase from Thermus caldophilus / Biotechnol. Lett 30:713~716
Wang L / 2012 / Immunosuppressive effects of ginsenoside-Rd on skin allograft rejection in rats / J. Surg. Res 176:267~274
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