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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제30권 제7호
- 발행연도
- 2020.1
- 수록면
- 1,037 - 1,043 (7page)
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Actinobacillus pleuropneumoniae (APP) is a causative agent of porcine pleuropneumonia. Therefore, the development of an effective vaccine for APP is necessary. Here, we optimized the culture medium and conditions to enhance the production yields of Apx toxins in APP serotype 1, 2, and 5 cultures. The use of Mycoplasma Broth Base (PPLO) medium improved both the quantity and quality of the harvested Apx toxins compared with Columbia Broth medium. Calcium chloride (CaCl2) was first demonstrated as a stimulation factor for the production of Apx toxins in APP serotype 2 cultures. Cultivation of APP serotype 2 in PPLO medium supplemented with 10 μg/ml of nicotinamide adenine dinucleotide (NAD) and 20 mM CaCl2 yielded the highest levels of Apx toxins. These findings suggest that the optimization of the culture medium and conditions increases the concentration of Apx toxins in the supernatants of APP serotype 1, 2, and 5 cultures and may be applied for the development of vaccines against APP infection.
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참고문헌 (33)
참고문헌 신청
Sassu EL / 2018 / Update on Actinobacillus pleuropneumoniae−knowledge, gaps and challenges / Transbound. Emerg. Dis. 65(Suppl 1):72~90
Liu J / 2007 / Potential use an Actinobacillus pleuropneumoniae double mutant strain ΔapxIICΔapxIVA as live vaccine that allows serological differentiation between vaccinated and infected animals / Vaccine 25:7696~7705
Mei L / 2006 / Study on the immunogenicity of N-terminal polypeptide of RTX toxin I of Actinobacillus pleuropneumoniae / Chin. J. Biotechnol 22:39~45
Bossé JT / 2018 / Proposal of serovars 17 and 18 of Actinobacillus pleuropneumoniae based on serological and genotypic analysis / Vet. Microbiol 217:1~6
Ramjeet M / 2008 / Actinobacillus pleuropneumoniae vaccines : from bacterins to new insights into vaccination strategies / Anim. Health. Res. Rev 9:25~45
Liu J / 2007 / Potential use an Actinobacillus pleuropneumoniae double mutant strain ΔapxIICΔapxIVA as live vaccine that allows serological differentiation between vaccinated and infected animals / Vaccine 25:7696~7705
Mei L / 2006 / Study on the immunogenicity of N-terminal polypeptide of RTX toxin I of Actinobacillus pleuropneumoniae / Chin. J. Biotechnol 22:39~45
Bossé JT / 2018 / Proposal of serovars 17 and 18 of Actinobacillus pleuropneumoniae based on serological and genotypic analysis / Vet. Microbiol 217:1~6
Ramjeet M / 2008 / Actinobacillus pleuropneumoniae vaccines : from bacterins to new insights into vaccination strategies / Anim. Health. Res. Rev 9:25~45
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