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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.1
- 수록면
- 429 - 438 (10page)
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초록· 키워드
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The emergence of multi-drug resistant, pathogenic methicillin-resistant Staphylococcus aureus (MRSA) is a threat to global health and has created a need for novel functional therapeutic agents. In this study, we evaluated the underlying mechanisms of the anti-MRSA effect of an azaphilone pigment, sclerotiorin (SCL) from Penicillium sclerotiorum. The antimicrobial activity of SCL was evaluated using agar disc diffusion, broth microdilution, time-kill assays and biophysical studies. SCL exhibits selective activity against Gram positive bacteria including MRSA (range, MIC = 128-1028 μg/ml) and exhibited rapid bactericidal action against MRSA with a > 4 log reduction in colony forming units within three hours of administration. Biophysical studies, using fluorescent probes and laser or electron microscopy, demonstrated a SCL dose-dependent alternation in membrane potential (62.6 ± 5.0.4% inhibition) and integrity (> 95 ± 2.3%), and the release of UV260 absorbing materials within 60 min (up to 3.2 fold increase, p < 0.01) of exposure. Further, SCL localized to the cytoplasm and hydrolyzed plasmid DNA. While in vitro checkerboard studies revealed that SCL potentiated the antimicrobial activity of topical antimicrobials such as polymixin, neomycin, and bacitracin (Fractional Inhibitory Concentration Index range, 0.26-0.37). Taken together these results suggest that SCL targets the membrane and DNA of MRSA to facilitate its anti- MRSA antimicrobial effect.
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참고문헌 (35)
참고문헌 신청
Stewart CM / 2002 / Staphylococcus aureus growth boundaries : moving mechanistic predictive mode is based on solute-specific effects / Appl. Environ. Microbiol 68:1864~1871
Tommasi R / 2015 / ESKAPEing the labyrinth of antibacterial discovery / Nat. Rev. Drug. Disc 14:529~542
Friedman M / 2015 / Antibiotic-resistant bacteria : prevalence in food and inactivation by food compatible compounds and plant extracts / J. Agric. Food. Chem 63:3805~3822
Osmanova N / 2010 / Azaphilones a class of fungal metabolites with diverse biological activities / Phytochem. Rev 9:315~342
Dong J / 2006 / New nematicidal azaphilones from the aquatic fungus Pseudohalonectria adversaria YMF1.01019 / FEMS. Microbiol. Lett 264:65~69
Tommasi R / 2015 / ESKAPEing the labyrinth of antibacterial discovery / Nat. Rev. Drug. Disc 14:529~542
Friedman M / 2015 / Antibiotic-resistant bacteria : prevalence in food and inactivation by food compatible compounds and plant extracts / J. Agric. Food. Chem 63:3805~3822
Osmanova N / 2010 / Azaphilones a class of fungal metabolites with diverse biological activities / Phytochem. Rev 9:315~342
Dong J / 2006 / New nematicidal azaphilones from the aquatic fungus Pseudohalonectria adversaria YMF1.01019 / FEMS. Microbiol. Lett 264:65~69
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