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논문 기본 정보

자료유형
학술저널
저자정보
Yi So Yeon (Bionanotechnology Research Center Korea Research Institute of Bioscience and Biotechnology (KRIBB) Daejeon 34141 Republic of Korea) Jeong Jinyoung (Environmental Disease Research Center Korea Research Institute of Bioscience and Biotechnology (KRIBB) Daejeon 34141 Republic of KoreaDepartment of Bioengineering KRIBB School University of Science an) Lee Wang Sik (Environmental Disease Research Center Korea Research Institute of Bioscience and Biotechnology (KRIBB) Daejeon 34141 Republic of Korea) Kwon Jungsun (BioNano Health Guard Research Center Daejeon 34141 Republic of Korea) Yoon Kyungah (Department of Clinical Pathology Daejeon Health Institute of Technology Daejeon 34504 Republic of Korea) Park Kyoungsook (Department of Biopharmacy Daejeon Health Institute of Technology Daejeon 34504 Republic of Korea)
저널정보
한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제33권 제5호
발행연도
2023.5
수록면
698 - 705 (8page)
DOI
10.4014/jmb.2301.01004

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Rapid diagnosis of methicillin-resistant Staphylococcus aureus (MRSA) is essential for guiding clinical treatment and preventing the spread of MRSA infections. Herein, we present a simple and rapid MRSA screening test based on the aggregation effect of mannose-binding lectin (MBL)-conjugated gold nanoparticles (AuNP), called the MRSA probe. Recombinant MBL protein is a member of the lectin family and part of the innate immune system. It can recognize wall teichoic acid (WTA) on the membrane of MRSA more specifically than that of methicillin-sensitive Staphylococcus aureus (MSSA) under optimized salt conditions. Thus, the MRSA probe can selectively bind to MRSA, and the aggregation of the probes on the surface of the target bacteria can be detected and analyzed by the naked eye within 5 min. To demonstrate the suitability of the method for real-world application, we tested 40 clinical S. aureus isolates (including 20 MRSA specimens) and recorded a sensitivity of 100%. In conclusion, the MRSA probe-based screening test with its excellent sensitivity has the potential for successful application in the microbiology laboratory.

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