지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.1
- 수록면
- 576 - 581 (6page)
이용수
초록· 키워드
오류제보하기
Dimethylation of the histone H3 protein at lysine residue 9 (H3K9) is mediated by euchromatin histone methyltransferase II (EHMT2) and results in transcriptional repression of target genes. Recently, chemical inhibition of EHMT2 was shown to induce various physiological outcomes, including endoplasmic reticulum stress-associated genes transcription in cancer cells. To identify genes that are transcriptionally repressed by EHMT2 during apoptosis, and cell stress responses, we screened genes that are upregulated by BIX-01294, a chemical inhibitor of EHMT2. RNA sequencing analyses revealed 77 genes that were upregulated by BIX-01294 in all four hepatic cell carcinoma (HCC) cell lines. These included genes that have been implicated in apoptosis, the unfolded protein response (UPR), and others. Among these genes, the one encoding the stress-response protein Ras-related GTPase C (RRAGC) was upregulated in all BIX-01294-treated HCC cell lines. We confirmed the regulatory roles of EHMT2 in RRAGC expression in HCC cell lines using proteomic analyses, chromatin immune precipitation (ChIP) assay, and small guide RNA-mediated loss-of-function experiments. Upregulation of RRAGC was limited by the reactive oxygen species (ROS) scavenger N-acetyl cysteine (NAC), suggesting that ROS are involved in EHMT2-mediated transcriptional regulation of stress-response genes in HCC cells. Finally, combined treatment of cells with BIX-01294 and 5- Aza-cytidine induced greater upregulation of RRAGC protein expression. These findings suggest that EHMT2 suppresses expression of the RRAGC gene in a ROS-dependent manner and imply that EHMT2 is a key regulator of stress-responsive gene expression in liver cancer cells.
목차
등록된 정보가 없습니다.
참고문헌 (20)
참고문헌 신청
Chen Z / 2017 / Epigenetic Regulation : A new frontier for biomedical engineers / Annu Rev Biomed Eng 19:195~219
현광범 / 2017 / Writing, erasing and reading histone lysine methylations / Experimental and Molecular Medicine 49:1~22
Herz HM / 2013 / SET for life : biochemical activities and biological functions of SET domaincontaining proteins / Trends Biochem Sci 38:621~639
Fritsch L / 2010 / A subset of the histone H3 lysine 9 methyltransferases Suv39h1, G9a, GLP, and SETDB1 participate in a multimeric complex / Mol Cell 37:46~56
Casciello F / 2015 / Functional role of G9a histone methyltransferase in cancer / Front Immunol 6:487
현광범 / 2017 / Writing, erasing and reading histone lysine methylations / Experimental and Molecular Medicine 49:1~22
Herz HM / 2013 / SET for life : biochemical activities and biological functions of SET domaincontaining proteins / Trends Biochem Sci 38:621~639
Fritsch L / 2010 / A subset of the histone H3 lysine 9 methyltransferases Suv39h1, G9a, GLP, and SETDB1 participate in a multimeric complex / Mol Cell 37:46~56
Casciello F / 2015 / Functional role of G9a histone methyltransferase in cancer / Front Immunol 6:487
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
BIX01294 inhibits EGFR signaling in EGFR-mutant lung adenocarcinoma cells through a BCKDHA-mediated reduction in the EGFR level
Experimental and Molecular Medicine
2021 .12
-
Epigenetic memory in gene regulation and immune response
BMB Reports
2019 .01
-
Regulation of cellular gene expression by nanomaterials
Nano Convergence
2018 .11
-
Unfolded Protein Response Monitoring System for Intuitive Identification of ER Stress in CHO Cells
한국생물공학회 학술대회
2021 .04
-
Development of Novel Suspending Cell Culture Substrates for ex vivo Cell-gene Therapy
한국생물공학회 학술대회
2018 .04
-
Histone tail cleavage as a novel epigenetic regulatory mechanism for gene expression
BMB Reports
2018 .01
-
Predicting Gene Expressions from Gene Expressions : Comparison between Lasso and Random Forest
한국정보과학회 학술발표논문집
2018 .06
-
Comparative global immune-related gene profiling of somatic cells, human pluripotent stem cells and their derivatives: implication for human lymphocyte proliferation
Experimental and Molecular Medicine
2017 .01
-
Development of a Deep Learning Model for Predicting the Effects of Cultivation Condition on Gene Expression Levels in Escherichia coli
한국생물공학회 학술대회
2021 .10
-
Deciphering the molecular mechanisms of epitranscriptome regulation in cancer
BMB Reports
2021 .01
-
A short guide to histone deacetylases including recent progress on class II enzymes
Experimental and Molecular Medicine
2020 .02
-
Gene Expression Mediating Messenger RNA Nanoparticles
한국고분자학회 학술대회 연구논문 초록집
2015 .10
-
Light-responsive Gene Delivery into a Single Living Cell
한국고분자학회 학술대회 연구논문 초록집
2023 .04
-
The aftermath of the interplay between the endoplasmic reticulum stress response and redox signaling
Experimental and Molecular Medicine
2021 .02
-
Modulation of Gene Expression using Synthetic Regulatory Small RNas in Escherichia coli
한국생물공학회 학술대회
2017 .04
-
Protein arginine methyltransferases: promising targets for cancer therapy
Experimental and Molecular Medicine
2021 .05
-
업무용 건물에너지 정보 호환 데이터 모델 개발
대한설비공학회 학술발표대회논문집
2016 .06
-
Single-cell analysis of salt-induced hypertensive mouse aortae reveals cellular heterogeneity and state changes
Experimental and Molecular Medicine
2021 .12
-
Photobiomodulation Facilitates Rat Cutaneous Wound Healing by Promoting Epidermal Stem Cells and Hair Follicle Stem Cells Proliferation
조직공학과 재생의학
2024 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0