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Organic wastes such as food waste (FW), livestock wastewater (LW), and sewage sludge (SWS) can produce hydrogen (H₂) by anaerobic acid fermentation. Expecially, FW which has high carbohydrate content produces H₂ and short chain fatty acids by indigenous H₂ producing microorganisms without adding inoculum, however H₂ production rate (HPR) and yield have to be improved to use a commercially available technology. In this study, LW was mixed to FW in different ratios (on chemical oxygen demand (COD) basis) as an auxiliary substrate. The mixture of FW and LW was pretreated at pH 2 using 6 N HCl for 12 h and then fermented at 37°C for 28 h. HPR of FW, 254 mL H₂/L/h, was increased with the addition of LW, however, mixing ratio of LW to FW was reversely related to HPR, exhibiting HPR of 737, 733, 599, and 389 mL H₂/L/h at the ratio of FW:LW=10:1, 10:2, 10:3, and 10:4 on COD basis, respectively. Maximum HPR and H₂ production yield of 737 H₂/L/h and 1.74 mol H₂/mol hexoseadded were obtained respectively at the ratio of FW:LW=10:1. Butyrate was the main organic acid produced and propionate was not detected throughout the experiment.

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