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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.3
- 수록면
- 246 - 253 (8page)
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초록· 키워드
오류제보하기
Background: Ginseng is one of the most valuable herbal supplements. It is challenging to perform quality control of ginseng products due to the diversity of bioactive saponins in their composition. Acid or alkaline hydrolysis is often used for the structural elucidation of these saponins and sugars in their side chains. Complete transformation of the original ginsenosides into their aglycones during the hydrolysis is one of the ways to determine a total saponin group content. The main hurdle of this approach is the formation of various by-products that was reported by many authors.
Methods: Separate HPLC assessment of the total protopanaxadiol, protopanaxatriol and ocotillol ginsenoside contents is a viable alternative to the determination of characteristic biomarkers of these saponin groups, such as ginsenoside Rf and pseudoginsenoside F<SUB>11</SUB>, which are commonly used for authentication of P. ginseng Meyer and P. quinquefolius L. samples respectively. Moreover, total ginsenoside content is an ideal aggregated parameter for standardization and quality control of ginseng-based medicines, because it can be directly applied for saponin dosage calculation.
Results: Different hydrolysis conditions were tested to develop accurate quantification method for the elucidation of total ginsenoside contents in herbal products. Linearity, limits of quantification, limits of detection, accuracy and precision were evaluated for the developed HPLC-MS method.
Conclusion: Alkaline hydrolysis results in fewer by-products than sugar elimination in acidic conditions. An equimolar response, as a key parameter for quantification, was established for several major ginsenosides. The developed approach has shown acceptable results in the analysis of several different herbal products.
Methods: Separate HPLC assessment of the total protopanaxadiol, protopanaxatriol and ocotillol ginsenoside contents is a viable alternative to the determination of characteristic biomarkers of these saponin groups, such as ginsenoside Rf and pseudoginsenoside F<SUB>11</SUB>, which are commonly used for authentication of P. ginseng Meyer and P. quinquefolius L. samples respectively. Moreover, total ginsenoside content is an ideal aggregated parameter for standardization and quality control of ginseng-based medicines, because it can be directly applied for saponin dosage calculation.
Results: Different hydrolysis conditions were tested to develop accurate quantification method for the elucidation of total ginsenoside contents in herbal products. Linearity, limits of quantification, limits of detection, accuracy and precision were evaluated for the developed HPLC-MS method.
Conclusion: Alkaline hydrolysis results in fewer by-products than sugar elimination in acidic conditions. An equimolar response, as a key parameter for quantification, was established for several major ginsenosides. The developed approach has shown acceptable results in the analysis of several different herbal products.
목차
ABSTRACT
1. Introduction
2. Materials and methods
3. Results and discussion
4. Conclusions
References
참고문헌 (21)
참고문헌 신청
Zhang H / 2017 / Sustained energy for enhanced human functions and activity / Academic Press :205~229
Li S / 2017 / Extraction and in vitro screening of potential acetylcholinesterase inhibitors from the leaves of Panax japonicas / J Chromatogr B 1061(1062):139~145
Wang CZ / 2016 / Red ginseng and cancer treatment / Chin J Nat Med 14:7~16
Laura Rojas Lorz / 2020 / Medicinal potential of Panax ginseng and its ginsenosides in atopic dermatitis treatment / Journal of Ginseng Research 44(1):8~13
Yang WZ / 2012 / A strategy for efficient discovery of new natural compounds by integrating orthogonal column chromatography and liquid chromatography/mass spectrometry analysis : its application in Panax ginseng, Panax quinquefolium and Panax notoginseng to characterize 437 potential new ginsenosides / Anal Chim Acta 739:56~66
Li S / 2017 / Extraction and in vitro screening of potential acetylcholinesterase inhibitors from the leaves of Panax japonicas / J Chromatogr B 1061(1062):139~145
Wang CZ / 2016 / Red ginseng and cancer treatment / Chin J Nat Med 14:7~16
Laura Rojas Lorz / 2020 / Medicinal potential of Panax ginseng and its ginsenosides in atopic dermatitis treatment / Journal of Ginseng Research 44(1):8~13
Yang WZ / 2012 / A strategy for efficient discovery of new natural compounds by integrating orthogonal column chromatography and liquid chromatography/mass spectrometry analysis : its application in Panax ginseng, Panax quinquefolium and Panax notoginseng to characterize 437 potential new ginsenosides / Anal Chim Acta 739:56~66
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