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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.8
- 수록면
- 475 - 479 (5page)
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Tegumental and excretory-secretory proteins are reported as diagnostic antigens for human opisthorchiasis. Rhophilin associated tail protein1-like (OvROPN1L) protein of Opisthorchis viverrini sperm tail showed potential as a diagnostic antigen. The OvROPN1L recombinant fragments were assayed for diagnostic antigenicity for human opisthorchiasis using indirect ELISA. The strongest antigenic region was a N-terminus peptide of M1 - P56. One synthetic peptide (P1, L3-Q13) of this region showed the highest antigenicity to opisthorchiasis. Sera from other parasitic infections including Strongyloides stercoralis, hookworm, Taenia spp, minute intestinal flukes, Paragonimus spp showed lower reactivity to P1. Peptide P1 is located in the disordered N-terminus of ROPN1L supporting its suitability as linear epitope. In the Platyhelminthes the N-terminal sequence of ROPN1L is diverging with taxonomic distance further suggesting that peptide P1 has potential as diagnostic tool in the genus Opisthorchis/Clonorchis. It should be further evaluated in combination with peptides derived from other O. viverrini antigens to increase its diagnostic power.
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참고문헌 (15)
참고문헌 신청
Sripa B / 2011 / Opisthorchiasis and opisthorchis-associated cholangiocarcinoma in Thailand and Laos / Acta Trop 120(suppl):158~168
Umesha KR / 2008 / Opisthorchis viverrini : detection by polymerase chain reaction(PCR)in human stool samples / Exp Parasitol 120:353~356
Sato M / 2010 / Copro-DNA diagnosis of Opisthorchis viverrini and Haplorchis taichui infection in an endemic area of Lao PDR / Se Asian J Trop Med 41:28~35
Duenngai K / 2013 / Diagnosis of early infection and post chemotherapeutic treatment by copro-DNA detection in experimental opisthorchiasis / Parasitol Res 112:271~278
Worasith C / 2015 / Advances in the Diagnosis of Human Opisthorchiasis : development of Opisthorchis viverrini antigen detection in Urine / PLoS Negl Trop Dis 9:e0004157
Umesha KR / 2008 / Opisthorchis viverrini : detection by polymerase chain reaction(PCR)in human stool samples / Exp Parasitol 120:353~356
Sato M / 2010 / Copro-DNA diagnosis of Opisthorchis viverrini and Haplorchis taichui infection in an endemic area of Lao PDR / Se Asian J Trop Med 41:28~35
Duenngai K / 2013 / Diagnosis of early infection and post chemotherapeutic treatment by copro-DNA detection in experimental opisthorchiasis / Parasitol Res 112:271~278
Worasith C / 2015 / Advances in the Diagnosis of Human Opisthorchiasis : development of Opisthorchis viverrini antigen detection in Urine / PLoS Negl Trop Dis 9:e0004157
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UCI(KEPA) : I410-ECN-0101-2020-513-001291201