Effect of Temperature on the Killing of Opisthorchis viverrini Eggs In Vitro

Parichart Boueroy; Kunyarat Duenngai; Chatanun Eamudomkarn; Panupan Sripan; Thidarut Boonmars; Benjamabhorn Pumhirunroj; Atchara Artchayasawat; Jiraporn Songsri; Kanpicha Chomphumee; Panaratana Rattanasuwan; Porntip Laummaunwai; Sukhonthip Khueangchiangkhwang; Sirintip Boonjaraspinyo

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저자정보: Parichart Boueroy (Khon Kaen University) Kunyarat Duenngai (Khon Kaen University) Chatanun Eamudomkarn (Khon Kaen University) Panupan Sripan (Khon Kaen University) Thidarut Boonmars (Khon Kaen University) Benjamabhorn Pumhirunroj (Khon Kaen University) Atchara Artchayasawat (Khon Kaen University) Jiraporn Songsri (Khon Kaen University) Kanpicha Chomphumee (Khon Kaen University) Panaratana Rattanasuwan (Khon Kaen University) Porntip Laummaunwai (Khon Kaen University) Sukhonthip Khueangchiangkhwang (Khon Kaen University) Sirintip Boonjaraspinyo (Khon Kaen University)

저널정보: 대한기생충학열대의학회 Parasites, Hosts and Diseases The Korean Journal of Parasitology Vol.57 No.1

발행연도: 2019.2

수록면: 49 - 53 (5page)

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Contaminated liver fluke egg in the environment has led to the high prevalence of human opisthorchiasis associated with cholangiocarcinoma in Southeast Asia. To find the effective lessening methods of Opisthorchis viverrini eggs in the contaminated environment, we investigated the temperature conditions for killing of these trematode eggs in vitro. Numerous O. viverrini eggs were obtained in the proximal part of uteri of adult worms from experimental hamsters. Mature eggs with miracidium were allocated by experimental groups (2 control: positive and negative and 4 treatment: 50, 60, 70, and 80°C) with 0.85% saline, and treated by the experimental plan. Eggs in each experimental groups were observed under the confocal microscope after stain with Propidium Iodide (PI) to evaluate the effect of temperatures. Eggs in 70 and 80°C groups were all killed after over 10 min heated. Majority of eggs in 60°C (10, 15, and 30 min heated), 70 and 80°C (5 min heated) groups were inactivated. However in 50°C group, below half of eggs were to be killed in all time lapse (10, 15 and 30 min). In order to prevent O. viverrini infection and cholangiocarcinoma, direct treatment of sewage by heating at 70 or 80°C at least 10 min is essential. Therefore, treatment of O. viverrini eggs at a high temperature is a potential method for controlling egg contamination in sewage.

#Opisthorchis viverrini #egg #heating #temperature #treatment

참고문헌 (33)

참고문헌 신청

Sripa B / 2010 / Food-borne trematodiases in Southeast Asia : epidemiology, pathology, clinical manifestation and control / Adv Parasitol 72:305~350

Sripa B / 2008 / Cholangiocarcinoma : lessons from Thailand / Curr Opin Gastroenterol 24:349~356

World Health Organization / 1995 / Control of foodborne trematode infections. Report of a WHO Study Group :1~157

Kaewkes S / 2003 / Taxonomy and biology of liver flukes / Acta Trop 88:177~186

Jongsuksuntigul P / 2003 / Opisthorchiasis control in Thailand / Acta Trop 88:229~232

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