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논문 기본 정보

자료유형
학술저널
저자정보
Shin, Young-Shin (Korean Intellectual Property Office, Governmental Complex) Chung, Hee-Sun (National Institute of Scientific Investigation) Pyo, Myoung-Yun (College of Pharmacy, Sookmyung Women's University) Baeck, Seung-Kyung (Central District Office, National Institute of Scientific Investigation)
저널정보
대한약학회 Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea 제30권 제2호
발행연도
2007.1
수록면
235 - 239 (5page)

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초록· 키워드

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The most important step for Paraquat analysis in post-mortem human blood (PMB) is its extraction from the specimens, as Paraquat is insoluble in organic solvents due to its ionic form. The most common extraction method, solid phase extraction (SPE), has been used for the extraction of Paraquat from PMB. However, SPE procedures are somewhat time-consuming, and resulted in unsatisfactory recovery in our laboratory. Therefore, SPE procedures, with five extraction solvents for the liquid-liquid extraction (LLE) of paraquat in PMB, were compared using HPLC, and the chloroform-ethanol (7:3, v/v) solvent mixture in human whole blood most effective. The recoveries of Paraquat using the 7:3 solvent mixture in human whole blood samples, which were already spiked with paraquat standards (1.25, 2.10 and 4.21 ${\mu}$g/mL) averaged 98.20, 105.71 and 99.40%, but the recoveries from the SPE were about 74.29, 78.50 and 80.10%, respectively. Linearity was obtained for the range of Paraquat standards, with a correlation coefficient; r$^2$>0.999. The limit of detection (LOD, with S/N${\ge}$3) and limit of quantitation (LOQ, with S/N${\ge}$10) were 0.01 and 0.05 ${\mu}$g/mL, respectively. The extraction method was successfully applied to seven real post-mortem cases involving paraquat poisoning.

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