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논문 기본 정보

자료유형
학술저널
저자정보
Shin, Jin-Ho (Department of Food Science and Biotechnology of Animal Resources, Konkuk University) Park, Jung-Min (Department of Food Science and Biotechnology of Animal Resources, Konkuk University) Kim, Ha-Jung (Department of Food Science and Biotechnology of Animal Resources, Konkuk University) Ahn, Jang-Hyuk (KOTITI Testing & Research Institute) Kwak, Byung-Man (Food Safety Center, Research and Development Institute, Namyang Dairy Co., Ltd.) Kim, Jin-Man (Department of Food Science and Biotechnology of Animal Resources, Konkuk University)
저널정보
한국축산식품학회 한국축산식품학회지 한국축산식품학회지 제35권 제4호
발행연도
2015.1
수록면
466 - 472 (7page)

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A rapid and simple analytical method, using liquid chromatography tandem mass spectrometry (LC-MS/MS), was developed to detect myo-inositol (MI) in infant formulas. For protein removal: acid hydrolysis and lipid removal through organic solvent extraction. The operating conditions for instrumental analysis were determined based on previously reported analogous methods that used LC-MS/MS. Quantitative analysis was used for the detection limit test, infant formula recovery test, and standard reference material (SRM) 1849a to verify the validity of our LC-MS/MS analytical method, which was developed to quantify MI. For validation, the results of our method were compared with the results of quantitative analyses of certified values. The test results showed that the limit of detection was 0.05 mg/L, the limit of quantitation was 0.17 mg/L, and the method detection limit was 17 mg/kg. The recovery test exhibited a recovery between 98.07-98.43% and a relative standard deviation between 1.93-2.74%. Therefore, the result values were good. Additionally, SRM 1849a was measured to have an MI content of 401.84 mg/kg and recovery of 98.25%, which is comparable to the median certified value of 409 mg/kg. From the aforementioned results, we judged that the instrumental analysis conditions and preparation method used in this study were valid. The rapid analytical method developed herein could be implemented in many laboratories that seek to save time and labor.

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