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논문 기본 정보

자료유형
학술저널
저자정보
Lee, Jung-Hoon (Department of Food Science and Biotechnology of Animal Resources, Konkuk University) Shin, Jin-Ho (Department of Food Science and Biotechnology of Animal Resources, Konkuk University) Park, Jung-Min (Department of Food Science and Biotechnology of Animal Resources, Konkuk University) Kim, Ha-Jung (Department of Food Science and Biotechnology of Animal Resources, Konkuk University) Ahn, Jang-Hyuk (KOTITI Testing & Research Institute) Kwak, Byung-Man (Food Safety Center, Research and Development Institute, Namyang Dairy Co., Ltd.) Kim, Jin-Man (Department of Food Science and Biotechnology of Animal Resources, Konkuk University)
저널정보
한국축산식품학회 한국축산식품학회지 한국축산식품학회지 제35권 제6호
발행연도
2015.1
수록면
765 - 771 (7page)

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The development of a sample preparation method and optimization of the analytical instrumentation conditions were performed for the determination of the vitamin B<sub>12</sub> content in emulsified baby foods sold on the Korea market. After removal of the milk protein and fats by chloroform extraction and centrifugation, the vitamin B<sub>12</sub> was water extracted from the sample. Following filtration of the solution through a nylon filter, the water-soluble extract was purified by solid-phase extraction using a Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS). The solution eluted from the cartridge was dried under a stream of nitrogen gas and reconstituted with 1 mL of water. The sample solution was injected into an LC-MS/MS system after optimizing the mobile phase for vitamin B<sub>12</sub> detection. The calibration curve showed good linearity with the coefficient of correlation (r<sup>2</sup>) value of 0.9999. The limit of detection was 0.03 &#xB5;g/L and the limit of quantitation was 0.1 &#xB5;g/L. The method of detection limit was 0.02 &#xB5;g/kg. The vitamin B<sub>12</sub> recovery from a spiking test was 99.62% for infant formula and 99.46% for cereal-based baby food. The sample preparation method developed in this study would be appropriate for the rapid determination of the vitamin B<sub>12</sub> content in infant formula and baby foods with emulsified milk characteristics. The ability to obtain stable results more quickly and efficiently would also allow governments to exercise a more extensive quality control inspection and monitoring of products expected to contain vitamin B<sub>12</sub>. This method could be implemented in laboratories that require time and labor saving.

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