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논문 기본 정보

자료유형
학술저널
저자정보
Hwang, In-Sul (Animal Biotechnology Division, National Institute of Animal Science, RDA) Lee, Seung-Chan (Animal Biotechnology Division, National Institute of Animal Science, RDA) Kim, Sung Woo (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA) Kwon, Dae-Jin (Animal Biotechnology Division, National Institute of Animal Science, RDA) Park, Mi-Ryung (Animal Biotechnology Division, National Institute of Animal Science, RDA) Yang, Hyeon (Animal Biotechnology Division, National Institute of Animal Science, RDA) Oh, Keon Bong (Animal Biotechnology Division, National Institute of Animal Science, RDA) Ock, Sun-A (Animal Biotechnology Division, National Institute of Animal Science, RDA) Woo, Jae-Seok (Animal Biotechnology Division, National Institute of Animal Science, RDA) Im, Gi-Sun (Animal Biotechnology Division, National Institute of Animal Science, RDA) Hwang, Seongsoo (Animal Biotechnology Division, National Institute of Animal Science, RDA)
저널정보
한국동물번식학회 한국수정란이식학회지 한국수정란이식학회지 제32권 제2호
발행연도
2017.1
수록면
53 - 58 (6page)

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It is very difficult to get the information about semen quality analysis in transgenic pigs because of limited numbers and research facilities. Therefore, in the present study, we analyzed the semen quality of transgenic boars generated for xenotransplantation research. Briefly, the semen samples were collected from 5 homozygous ${\alpha}1,3$-Galactosyltransferase knock-out ($GalT^{-/-}$) transgenic boars and immediately transported to the laboratory. These semen samples were decupled with DPBS and conducted to analyze semen parameters by a computer-assisted semen analysis (CASA) system. The boar semen were examined all 12 parameters such as total motility (TM), curvilinear velocity (VCL), straight line velocity (VSL), average path velocity (VAP), and hyperactivated (HYP), etc. In results, among the 5 $GalT^{-/-}$ boars, three boars (#134, 144, and 170) showed normal range of semen parameters, but #199 and 171 boars showed abnormal ranges of semen parameters according to standard ranges of semen parameters. Unfortunately, #171 boar showed azoospermia symptom with rare sperm counts in the original semen. Conclusively, assessment of semen parameters by CASA system is useful to pre-screening of reproductively healthy boar prior to natural mating and artificial insemination for multiplication and breeding.

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