메뉴 건너뛰기
.. 내서재 .. 알림
소속 기관/학교 인증
인증하면 논문, 학술자료 등을  무료로 열람할 수 있어요.
한국대학교, 누리자동차, 시립도서관 등 나의 기관을 확인해보세요
(국내 대학 90% 이상 구독 중)
로그인 회원가입 고객센터 ENG
주제분류

추천
검색
질문

논문 기본 정보

자료유형
학술저널
저자정보
Adikari Arachchige Dilki Indrachapa Adikari (Sunchon National University) Young-Joo Yi (Sunchon National University)
저널정보
충남대학교 농업과학연구소 Korean Journal of Agricultural Science Korean Journal of Agricultural Science Vol.50 No.1
발행연도
2023.3
수록면
87 - 97 (11page)

이용수

표지
📌
연구주제
📖
연구배경
🔬
연구방법
🏆
연구결과
AI에게 요청하기
추천
검색
질문

초록· 키워드

오류제보하기
Fipronil sulfone (FS) is known to adversely affect the organs and organ systems of all living organisms exposed to this substance. The present study was performed to examine the effects of FS on the fertilizing capacity of boar spermatozoa. The boar sperm samples were subjected to varying concentrations of FS (10 - 200 μM) for two different incubation times (30 min and 2 hrs). Sperm motility, viability, acrosome integrity, and production of intracellular reactive oxygen species (ROS) were then evaluated. Real-time PCR was conducted to analyze the RNA expression of ODF2, ZPBP2, HSPA8, and AKAP4, which are associated with the integrity of the sperm fibrous sheath and sperm movement. A dose-dependent motility reduction was observed in sperm exposed to FS during both incubation periods compared to the controls. Higher percentages of viable sperm were observed in the controls, while viability was significantly decreased in sperm incubated with 20 - 200 μM FS. Acrosomes were damaged in sperm incubated with 200 μM FS after 30 min of incubation. Higher levels of intracellular reactive oxygen species (ROS) were produced in sperm exposed to FS after 2 hrs of incubation. Also, the mRNA expression levels of ODF2, ZPBP2, and AKAP4, which are associated with fertilizing capacity, were downregulated in sperm exposed to FS compared to the controls. Direct exposure to metabolites of specific pesticides such as fipronil affects male fertility due to their effects on sperm characteristics. It is thus necessary to screen metabolites that cause reproductive physiotoxicity to protect health and prevent sub-/infertility in animals.

목차

Abstract
Introduction
Materials and Methods
Results and Discussion
Conclusion
References

참고문헌 (0)

참고문헌 신청

함께 읽어보면 좋을 논문

논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!

이 논문의 저자 정보

최근 본 자료

전체보기

댓글(0)

0