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논문 기본 정보

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저자정보
Kim, Eun-Jung (Department of Dental Anesthesia and Pain Medicine, School of Dentistry, Pusan National University, Dental Research Institute) Kim, Hyung Joon (Department of Oral Physiology, School of Dentistry, Pusan National University) Baik, Seong Wan (Department of Anesthesia and Pain Medicine, School of Medicine, Pusan National University) Kim, Kyung-Hoon (Department of Anesthesia and Pain Medicine, School of Medicine, Pusan National University) Ryu, Sie Jeong (Department of Anesthesia and Pain Medicine, College of Medicine, Kosin University) Kim, Cheul-Hong (Department of Dental Anesthesia and Pain Medicine, School of Dentistry, Pusan National University, Dental Research Institute) Shin, Sang-Wook (Department of Anesthesia and Pain Medicine, School of Medicine, Pusan National University)
저널정보
대한치과마취과학회 Journal of dental anesthesia and pain medicine Journal of dental anesthesia and pain medicine 제18권 제6호
발행연도
2018.1
수록면
349 - 359 (11page)

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Background: Propofol is an intravenous anesthetic which has antioxidant effects due to its similarity in molecular structure to ${\alpha}$-tocopherol. It has been reported that ${\alpha}$-tocopherol increases osteoclast fusion and bone resorption. Here, we investigated the effects of propofol on signaling pathways of osteoclastogenic gene expression, as well as osteoclastogenesis and bone resorption using bone marrow-derived macrophages (BMMs). Methods: BMMs were cultured with macrophage colony-stimulating factor (M-CSF) alone or M-CSF plus receptor activator of nuclear factor kappa B ligand (RANKL) in the presence of propofol ($0-50{\mu}M$) for 4 days. Mature osteoclasts were stained for tartrate-resistant acid phosphatase (TRAP) and the numbers of TRAP-positive multinucleated osteoclasts were counted. To examine the resorption activities of osteoclasts, a bone resorption assay was performed. To identify the mechanism of action of propofol on the formation of multinucleated osteoclasts, we focused on dendritic cell-specific transmembrane protein (DC-STAMP), a protein essential for pre-osteoclastic cell fusion. Results: Propofol increased the formation of TRAP-positive multinucleated osteoclasts. In addition, the bone resorption assay revealed that propofol increased the bone resorption area on dentin discs. The mRNA expression of DC-STAMP was upregulated most strongly in the presence of both RANKL and propofol. However, SB203580, a p38 inhibitor, significantly suppressed the propofol/RANKL-induced increase in mRNA expression of DC-STAMP. Conclusion: We have demonstrated that propofol enhances osteoclast differentiation and maturation, and subsequently increases bone resorption. Additionally, we identified the regulatory pathway underlying osteoclast cell-cell fusion, which was enhanced by propofol through p38-mediated DC-STAMP expression.
#Bone Resorption #Cell Fusion #Osteoclasts #Propofol #p38 MAP Kinase

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참고문헌 (33)

참고문헌 신청
Karsenty G / 2009 / Genetic control of bone formation / Annu Rev Cell Dev Biol 25:629~648 google schola Walsh MC / 2006 / Osteoimmunology : interplay between the immune system and bone metabolism / Annu Rev Immunol 24:33~63 google schola Mountzios G / 2007 / Abnormal bone remodeling process is due to an imbalance in the receptor activator of nuclear factor-kappaB ligand(RANKL)/osteoprotegerin(OPG)axis in patients with solid tumors metastatic to the skeleton / Acta Oncol 46:221~229 google schola Corral DA / 1998 / Dissociation between bone resorption and bone formation in osteopenic transgenic mice / Proc Natl Acad Sci USA 95:13835~13840 google schola Theill LE / 2002 / RANK-L and RANK:T cells, bone loss, and mammalian evolution / Annu Rev Immunol 20:795~823 google schola

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