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논문 기본 정보

자료유형
학술저널
저자정보
Kim, Hong-Seok (KU Center for Food Safety and College of Veterinary Medicine, Konkuk University) Chon, Jung-Whan (KU Center for Food Safety and College of Veterinary Medicine, Konkuk University) Kim, Hyunsook (Dept. of Food & Nutrition, College of Human Ecology, Hanyang University) Kim, Dong-Hyeon (KU Center for Food Safety and College of Veterinary Medicine, Konkuk University) Yim, Jin-Hyuk (KU Center for Food Safety and College of Veterinary Medicine, Konkuk University) Song, Kwang-Young (KU Center for Food Safety and College of Veterinary Medicine, Konkuk University) Kang, Il-Byung (KU Center for Food Safety and College of Veterinary Medicine, Konkuk University) Kim, Young-Ji (KU Center for Food Safety and College of Veterinary Medicine, Konkuk University) Lee, Soo-Kyung (KU Center for Food Safety and College of Veterinary Medicine, Konkuk University) Seo, Kun-Ho (KU Center for Food Safety and College of Veterinary Medicine, Konkuk University)
저널정보
한국유가공기술과학회 Journal of Milk Science and Biotechnology(한국유가공기술과학회지) 한국유가공학회지 제33권 제4호
발행연도
2015.1
수록면
271 - 275 (5page)

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Colloidal semiconductor CdSe-ZnS core-shell nanocrystal quantum dot (Qdot) are luminescent inorganic fluorophores that show potential to overcome some of the functional limitations encountered with organic dyes in fluorescence labeling applications. Salmonella Enteritidis has emerged as a major cause of human salmonellosis worldwide since the 1980s. A rapid, specific, and sensitive method for the detection of Salmonella Enteritidis was developed using Qdot as a fluorescence marker coupled with immunomagnetic separation. Magnetic beads coated with anti-Salmonella Enteritidis antibodies were employed to selectively capture the target bacteria, and biotin-conjugated anti-Salmonella antibodies were added to form sandwich immune complexes. After magnetic separation, the immune complexes were labeled with Qdot via biotin-streptavidin conjugation, and fluorescence measurement was carried out using a fluorescence measurement system. The detection limit of the Qdot method was a Salmonella Enteritidis concentration of $10^3$ colony-forming units (CFU)/mL, whereas the conventional fluorescein isothiocyanate-based method required over $10^5CFU/mL$. The total detection time was within 2 h. In addition to the potential for general nanotechnology development, these results suggest a new rapid detection method of various pathogenic bacteria from a complex food matrix.

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