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자료유형
학술저널
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Sohn, Sung-Hwa (Department of Physiology, College of Oriental Medicine, KyungHee University) Ko, Eun-Jung (Department of Physiology, College of Oriental Medicine, KyungHee University) Kim, Yang-Seok (Department of Physiology, College of Oriental Medicine, KyungHee University) Shin, Min-Kyu (Department of Physiology, College of Oriental Medicine, KyungHee University) Hong, Moo-Chang (Department of Physiology, College of Oriental Medicine, KyungHee University) Bae, Hyun-Su (Department of Physiology, College of Oriental Medicine, KyungHee University)
저널정보
대한독성유전단백체학회 Molecular & cellular toxicology Molecular & cellular toxicology 제4권 제3호
발행연도
2008.1
수록면
205 - 210 (6page)

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Recent studies suggest that activated microglial cells play an essential role in the inflammatory responses and neurodegenerative disorders such as Alzheimer’s and Parkinson’s disease. This study was conducted to evaluate the protective mechanisms of Asiasarum sieboldi (AS) on LPS-induced activation of BV-2 microglial cells. The effects of AS on gene expression profiles in activated BV-2 microglial cells were evaluated using microarray analysis. BV-2 microglial cells were cultured in a 100 mm dish ($1{\times}10^7$/mL) for 24 h and then pretreated with 1 ${\mu}g$/mL AS or left untreated for 30 min. Next, 1 ${\mu}g$/mL LPS was added to the samples and the cells were reincubated at $37^{\circ}C$ for 30 min and 1 hr. The gene expression profiles of the BV-2 microglial cells varied depending on the AS. The microarray analysis revealed that MAPK signaling pathway-related genes were downregulated in AS-treated BV-2 microglial cells. AS can affect the neuroinflammatory-related pathway such as MAPK signaling pathway in activated BV-2 microglial cells.

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