지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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We report the existence of new type of phosphatidylcholine-hydrolyzing phospholipase D (PLD), which has been characterized and partially purified in the scuticociliate, Uronema marinum. The enzyme from partial purification showed that it was existed in membrane fraction and was a neutral PLD, which catalyzed both transphosphatidylation and hydrolysis reaction. The activity of partially purified membrane-bound PLD was also found to be optimal at pH 7.0-7.5 for 2 hours at 37℃ and depended strictly on the presence of Ca2+ (2.5 mM) and Mg2+ (1.6 mM). Immunoblot analysis indicated that the enzyme was distinct from hPLD1 (human PLD1) and hPLD2 (human PLD2) because it was not recognized by a polyclonal antibody raised to the 12 terminal amino acid of these enzymes. We also found that the membrane-bound PLD is a PIP2-dependent PLD and that GTP-binding proteins are not implicated in the regulation of this enzyme: This enzyme activity is markedly stimulated by phosphatidylinositol 4,5-bisphosphate (PIP2) but not by the small G-protein Arf and GTPrS. In addition, this enzyme was capable of hydrolyzing phosphatidylcholine (PC) but not phosphatidylethanolamine (PE), implying that PC was a preferred substrate.
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참고문헌 (40)
참고문헌 신청
Amsterdam, A. / 1994 / Role of phospholipase-D and phosphatidic acid in mediating gonadotropin-releasing hormone-induced inhibition of preantral granulose cell differentiation / Endocrinol 135:1205~1211
Banno, Y. / 1997 / Nuclear ADP-ribosylation factor (ARF) and oleate-dependent phospholipase D (PLD) in rat liver cells / increase of ARF-dependent PLD activity in regenerating liver cells. J. Biol. Chem 272:5208~5213
Billah, M. M. / 1993 / Phospholipase D and cell signaling / Curr. Opin. Immunol 5:114~123
Blum, J. J. / 2001 / hospholiase D is present in Leishmania donovani and its activity increases in response to acute osmotic stress / J. Eukaryot. Microbiol 48:102~110
Brown, H. A. / 1993 / ADP-ribosylation factor, a small GTP-dependent regulatory protein, stimulates phospholipase activity / Cell 75:1137~1144
Banno, Y. / 1997 / Nuclear ADP-ribosylation factor (ARF) and oleate-dependent phospholipase D (PLD) in rat liver cells / increase of ARF-dependent PLD activity in regenerating liver cells. J. Biol. Chem 272:5208~5213
Billah, M. M. / 1993 / Phospholipase D and cell signaling / Curr. Opin. Immunol 5:114~123
Blum, J. J. / 2001 / hospholiase D is present in Leishmania donovani and its activity increases in response to acute osmotic stress / J. Eukaryot. Microbiol 48:102~110
Brown, H. A. / 1993 / ADP-ribosylation factor, a small GTP-dependent regulatory protein, stimulates phospholipase activity / Cell 75:1137~1144
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