EphA2 Receptor Signaling Mediates Inflammatory Responses in Lipopolysaccharide-Induced Lung Injury

Hong, Ji Young; Shin, Mi Hwa; Chung, Kyung Soo; Kim, Eun Young; Jung, Ji Ye; Kang, Young Ae; Kim, Young Sam; Kim, Se Kyu; Chang, Joon; Park, Moo Suk

추천

검색

자료유형: 학술저널

저자정보: Hong, Ji Young (Division of Pulmonology, Department of Internal Medicine, Yonsei University College of Medicine) Shin, Mi Hwa (Division of Pulmonology, Department of Internal Medicine, Yonsei University College of Medicine) Chung, Kyung Soo (Division of Pulmonology, Department of Internal Medicine, Yonsei University College of Medicine) Kim, Eun Young (Division of Pulmonology, Department of Internal Medicine, Yonsei University College of Medicine) Jung, Ji Ye (Division of Pulmonology, Department of Internal Medicine, Yonsei University College of Medicine) Kang, Young Ae (Division of Pulmonology, Department of Internal Medicine, Yonsei University College of Medicine) Kim, Young Sam (Division of Pulmonology, Department of Internal Medicine, Yonsei University College of Medicine) Kim, Se Kyu (Division of Pulmonology, Department of Internal Medicine, Yonsei University College of Medicine) Chang, Joon (Division of Pulmonology, Department of Internal Medicine, Yonsei University College of Medicine) Park, Moo Suk (Division of Pulmonology, Department of Internal Medicine, Yonsei University College of Medicine)

저널정보: 대한결핵 및 호흡기학회 Tuberculosis and Respiratory Diseases 결핵 및 호흡기 질환 제78권 제3호

발행연도: 2015.1

수록면: 218 - 226 (9page)

이용수

📌

연구주제

📖

연구배경

🔬

연구방법

🏆

연구결과

초록· 키워드

오류제보하기

Background: Eph receptors and ephrin ligands have several functions including angiogenesis, cell migration, axon guidance, fluid homeostasis, oncogenesis, inflammation and injury repair. The EphA2 receptor potentially mediates the regulation of vascular permeability and inflammation in response to lung injury. Methods: Mice were divided into 3 experimental groups to study the role of EphA2 signaling in the lipopolysaccharide (LPS)-induced lung injury model i.e., IgG+phosphate-buffered saline (PBS) group (IgG instillation before PBS exposure), IgG+LPS group (IgG instillation before LPS exposure) and EphA2 monoclonal antibody (mAb)+LPS group (EphA2 mAb pretreatment before LPS exposure). Results: EphA2 and ephrinA1 were upregulated in LPS-induced lung injury. The lung injury score of the EphA2 mAb+LPS group was lower than that of the IgG+LPS group ($4.30{\pm}2.93$ vs. $11.45{\pm}1.20$, respectively; p=0.004). Cell counts (EphA2 mAb+LPS: $11.33{\times}10^4{\pm}8.84{\times}10^4$ vs. IgG+LPS: $208.0{\times}10^4{\pm}122.6{\times}10^4$; p=0.018) and total protein concentrations (EphA2 mAb+LPS: $0.52{\pm}0.41mg/mL$ vs. IgG+LPS: $1.38{\pm}1.08mg/mL$; p=0.192) were decreased in EphA2 mAb+LPS group, as compared to the IgG+LPS group. In addition, EphA2 antagonism reduced the expression of phospho-p85, phosphoinositide 3-kinase $110{\gamma}$, phospho-Akt, nuclear factor ${\kappa}B$, and proinflammatory cytokines. Conclusion: This results of the study indicated a role for EphA2-ephrinA1 signaling in the pathogenesis of LPS-induced lung injury. Furthermore, EphA2 antagonism inhibits the phosphoinositide 3-kinase-Akt pathway and attenuates inflammation.

#Lipopolysaccharides #Lung Injury #EphA2 Protein

참고문헌 (0)

참고문헌 신청

참고문헌이 DBpia에서 서비스 중이라면, [참고문헌 신청]을 통해 등록해보세요

함께 읽어보면 좋을 논문

논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!

이 논문의 저자 정보

Hong, Ji Young

소속기관 Division of Pulmonary, Allergy and Critical Care Medicine, Department of Internal Medicine, Hallym U

주요연구분야 의약학 > 임상의학 > 내과학

논문수 2 이용수 1

Shin, Mi Hwa

소속기관 Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Institute of Ches

주요연구분야 의약학 > 임상의학 > 내과학

논문수 2 이용수 0

Chung, Kyung Soo

소속기관 Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Institute of Ches

주요연구분야 의약학 > 임상의학 > 내과학

논문수 5 이용수 3

Kim, Eun Young

소속기관 Department of Internal Medicine, Yonsei University College of Medicine

주요연구분야 의약학 > 임상의학 > 내과학

논문수 14 이용수 4

Jung, Ji Ye