Scabraside D Extracted from Holothuria scabra Induces Apoptosis and Inhibits Growth of Human Cholangiocarcinoma Xenografts in Mice

Assawasuparerk, Kanjana; Vanichviriyakit, Rapeepun; Chotwiwatthanakun, Charoonroj; Nobsathian, Saksit; Rawangchue, Thanakorn; Wittayachumnankul, Boonsirm

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자료유형: 학술저널

저자정보: Assawasuparerk, Kanjana (Department of Anatomy, Prince of Songkhla University Surat Thani Campus) Vanichviriyakit, Rapeepun (Department of Anatomy, Prince of Songkhla University Surat Thani Campus) Chotwiwatthanakun, Charoonroj (Nakhonsawan Campus, Mahidol University) Nobsathian, Saksit (Nakhonsawan Campus, Mahidol University) Rawangchue, Thanakorn (Center for Veterinary Diagnosis, Faculty of Veterinary Science) Wittayachumnankul, Boonsirm (Department of Anatomy, Prince of Songkhla University Surat Thani Campus)

저널정보: 아시아태평양암예방학회 Asian Pacific journal of cancer prevention : APJCP Asian Pacific journal of cancer prevention : APJCP 제17권 제2호

발행연도: 2016.1

수록면: 511 - 517 (7page)

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Scabraside D, a sulfated triterpene glycoside extract from sea cucumber Holothulia scabra, shows various biological activities, but effects on human cholangiocarcinoma cells have not previously been reported. In the present study, we investigated the activity of scabraside D against human cholangiocarcinoma (HuCCA) both in vitro and for tumor growth inhibition in vivo using a xenograft model in nude mice. Scabraside D ($12.5-100{\mu}g/mL$) significantly decreased the viability and the migration of the HuCCA cells in a dose-dependent manner, with 50% inhibitory concentration (IC50) of $12.8{\pm}0.05{\mu}g/mL$ at 24 h. It induced signs of apoptotic cells, including shrinkage, pyknosis and karyorrhetic nuclei and DNA fragmentation on agarose gel electrophoresis. Moreover, by quantitative real-time PCR, scabraside D effectively decreased Bcl-2 while increasing Bax and Caspase-3 gene expression levels suggesting that the scabraside D could induce apoptosis in HuCCA cells. In vivo study demonstrated that scabraside D (1 mg/kg/day, i.p. for 21 days) significantly reduced growth of the HuCCA xenografts without adverse effects on the nude mice. Conclusively, scabraside D induced apoptosis in HuCCA cells and reduced the growth of HuCCA xenographs model. Therefore, scabraside D may have potential as a new therapeutic agent for cholangiocarcinoma.

#Scabraside D #Holothuria scabra #cholangiocarcinoma #apoptosis #sea cucumber extract

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