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자료유형
학술저널
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Chomean, Sirinart (Department of Medical Technology, Faculty of Allied Health Sciences, Thammasat University) Sukanto, Tanawan (Department of Medical Technology, Faculty of Allied Health Sciences, Thammasat University) Piemsup, Arreya (Department of Medical Technology, Faculty of Allied Health Sciences, Thammasat University) Chaiya, Jirattikan (Thammasat Infertility Center, Thammasat University Hospital, Thammasat University) Saenguthai, Kolunya (Medical laboratory, Thammasat University Hospital, Thammasat University) Kaset, Chollanot (Department of Medical Technology, Faculty of Allied Health Sciences, Thammasat University)
저널정보
대한생식의학회 Clinical and experimental reproductive medicine : CERM Clinical and experimental reproductive medicine : CERM 제46권 제2호
발행연도
2019.1
수록면
60 - 66 (7page)

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Objective: To compare black rice (Oryza sativa L) extract with three different staining methods for human sperm head assessment. Methods: Semen samples were collected from 34 volunteers. Four smears of each ejaculate were prepared for staining using the rapid Papanicolaou (PAP) stain, SpermBlue, DipQuick, and black rice extract. The percentage of defective sperm heads (mean${\pm}$standard deviation) was compared. Results: Black glutinous rice extract, a natural dye, was used instead of hematoxylin to stain the nuclei of the sperm heads. The percentage of defective sperm heads showed a significant difference between black rice extract and DipQuick (p= 0.000). In contrast, black rice extract and rapid PAP showed no statistically significant difference (p= 0.974). A strong correlation (r = 0.761) was found between the findings obtained using rapid PAP and black rice extract. In contrast, a weak correlation (r = 0.248) was obtained between DipQuick and black rice extract for the percentage of defective sperm heads. Conclusion: The results showed good agreement and a strong correlation between the rapid PAP and black rice extract stains. The advantages of black rice extract as a novel substitute for hematoxylin for nuclear staining include ease of preparation, local availability, and favorable nuclear staining properties. Further studies could also focus on comparing staining techniques in clinical samples.

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