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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 327 - 332 (6page)
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Phenylalanine ammonia-lyase (PAL) gene is known to be expressed in plants, and is involved in the differentiation, growth and synthesis of secondary metabolites. However, its expression in fungi remains to be explored. To understand its expression in mushroom fungi, the PAL gene of the edible mushroom Flammulina velutipes (Fvpal) was cloned and characterized. The cloned Fvpal consists of 2,175 bp, coding for a polypeptide containing 724 amino acids and having 11 introns. The translated amino acid sequence of Fvpal shares a high identity (66%) with that of ectomycorrhizal fungus Tricholoma matsutake. Distinctively, the Fvpal expression in the mycelium was higher in minimal medium supplemented with L-tyrosine than with other aromatic amino acids. During cultivation of the mushroom on sawdust medium, Fvpal expression in the fruit body correspondingly increased as the mushroom grew. In the fruiting body, Fvpal was expressed more in the stipe than in the pileus. These results suggest that F. velutipes PAL activity differs in the different organs of the mushroom. Overall, this is first report to show that the PAL gene expression is associated with mushroom growth in fungi.
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참고문헌 (22)
참고문헌 신청
Power DM / 1965 / Biosynthesis of phenolic acids by certain wood-destroying Basidiomycetes / Can J Biochem 43:1397~1407
Moore K / 1967 / Towers GH. Degradation of phenylalanine and tyrosine by Basidiomycetes / Life Sci 6:2629~2633
Yamada S / 1981 / Production of L-phenylalanine from trans-cinnamic acid with Rhodotorula glutinis containing L-phenylalanine ammonialyase activity / Appl Environ Microbiol 42:773~778
Moore K / 1968 / Degradation of phenylalanine and tyrosine by Sporobolomyces roseus / Biochem J 106:507~514
Leal GA / 2010 / Searching for Moniliophthora perniciosa pathogenicity genes / Fungal Biol 114:842~854
Moore K / 1967 / Towers GH. Degradation of phenylalanine and tyrosine by Basidiomycetes / Life Sci 6:2629~2633
Yamada S / 1981 / Production of L-phenylalanine from trans-cinnamic acid with Rhodotorula glutinis containing L-phenylalanine ammonialyase activity / Appl Environ Microbiol 42:773~778
Moore K / 1968 / Degradation of phenylalanine and tyrosine by Sporobolomyces roseus / Biochem J 106:507~514
Leal GA / 2010 / Searching for Moniliophthora perniciosa pathogenicity genes / Fungal Biol 114:842~854
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