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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국응용생명화학회 Journal of applied biological chemistry Journal of applied biological chemistry 제58권 제2호
- 발행연도
- 2015.1
- 수록면
- 97 - 100 (4page)
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초록· 키워드
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The thermostable pectinase gene TmPec isolated from Thermotoga maritima was introduced into the NdeI site of pRSET-B vector and expressed in its intact form in Escherichia coli BL21. The overexpressed intact form of pectinase (TmPecN protein) was partially purified by heat-denaturation procedure. TmPecN showed the highest activity between 85 and $95^{\circ}C$, and at approximately pH 6.5. Enzyme activity was stably maintained at temperatures below $85^{\circ}C$. In the presence of $Ca^{2+}$, pectinase activity of TmPecN increased to 128.4% of normal level. In contrast, $Ba^{2+}$, $Zn^{2+}$, and $Mn^{2+}$ strongly inhibited TmPecN activity. We conclude that the biochemical properties of the intact form of TmPecN are comparable to those of the recombinant protein TmPec reported previously.
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참고문헌 (18)
참고문헌 신청
Alkorta I / 1998 / Industrial applications of pectic enzymes: a review / Process Biochem 33:21~28
Beg QK / 2000 / Production and characterization of thermostable xylanase and pectinase from a Streptomyces sp. QG-11-3 / J Ind Microbiol Biotechnol 24:396~402
Blanco P / 1999 / Production of pectic enzymes in yeasts / FEMS Microbiol Lett 175:1~9
Bruhlmann F / 1995 / Purification and characterization of an extracellular pectate lyase from an Amylocota sp / Appl Environ Microbiol 61:3580~3585
Carpita NC / 1993 / Structural models of primary cell walls in flowering plants : consistency of molecular structure with the physical properties of the walls during growth / Plant Rev 3:1~30
Beg QK / 2000 / Production and characterization of thermostable xylanase and pectinase from a Streptomyces sp. QG-11-3 / J Ind Microbiol Biotechnol 24:396~402
Blanco P / 1999 / Production of pectic enzymes in yeasts / FEMS Microbiol Lett 175:1~9
Bruhlmann F / 1995 / Purification and characterization of an extracellular pectate lyase from an Amylocota sp / Appl Environ Microbiol 61:3580~3585
Carpita NC / 1993 / Structural models of primary cell walls in flowering plants : consistency of molecular structure with the physical properties of the walls during growth / Plant Rev 3:1~30
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