지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
- 수록면
- 543 - 551 (9page)
이용수
초록· 키워드
오류제보하기
Hepatitis C virus (HCV) non-structural 5A (NS5A) protein is associated with a wide variety of host signaling pathways by binding to C-terminal polyproline (PxxP) motifs of various proteins. In this study, we used yeast two-hybrid analysis and a GST pull-down assay to screen a novel NS5A interacting protein and elucidate the binding site and cellular signaling by focusing on recombinant human epidermal growth factor (rhEGF)-mediated ERK1/2 activation. Screening a liver cDNA library revealed that h-prune, a member of the DHH (Asp-His-His) protein superfamily, directly interacted with HCV NS5A C-terminus. In particular, a mutation of five proline amino acids to alanine in this region revealed that these two proteins produced strong interaction through this domain. It is known that h-prune possesses a highly conserved DHH motif, which has exopolyphosphatase activity that accelerates hydrolysis of inorganic polyphosphate. A time-chasing analysis after rhEGF treatment demonstrated that h-prune overexpression almost restored NS5A-mediated attenuation of ERK1/2 phosphorylation, but h-prune itself did not alter this signaling. Although the detailed mechanisms need to be clarified, this study demonstrates that h-prune interacts directly with the PxxP motif of the HCV NS5A C-terminus and that this binding alters the rhEGF-mediated ERK1/2 signaling cascade in liver cells.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Antioxidant activity and Quality Characteristics of Prune juice added with Medicinal Herbal Plants
한국식품영양과학회 학술대회발표집
2024 .10
-
Estimation on Production Potential of Agricultural By-product and Biochar in Jeollabuk-do Province
한국토양비료학회 학술발표회 초록집
2023 .10
-
Functional Analysis of PXXP Motif in Arabidopsis BAK1
한국원예학회 학술발표요지
2023 .05
-
Determination of the consensus sequence for FUS3-specific binding by protein binding microarray analysis
Applied Biological Chemistry
2015 .01
-
Vitamin C Regulates Osteoblastogenesis and Osteoclastogenesis through AKT and ERK Signaling Pathways
한국식품영양과학회 학술대회발표집
2019 .10
-
Current status of hepatitis C virus infection and countermeasures in South Korea
Epidemiology and Health
2017 .01
-
쌀단백질의 가치와 이용방법
식품과학과 산업
2019 .03
-
Expression and Purification of Extracellular Solute-Binding Protein (ESBP) in Escherichia coli, the Extracellular Protein Derived from Bifidobacterium longum KACC 91563
Food science of animal resources
2019 .01
-
Development of OECD Test Guideline for Human Androgen Receptor Binding Assay Using NanoBRET Protein-Protein Interaction System
한국식품영양과학회 학술대회발표집
2019 .10
-
Development of OECD Test Guide Line for Human Estrogen Receptor Binding Assay Using NanoBRET Protein-Protein Interaction System
한국식품영양과학회 학술대회발표집
2018 .10
-
배전정지 바이오차의 시용이 작물 생육 및 토양이화학성에 미치는 영향
한국토양비료학회 학술발표회 초록집
2017 .05
-
Optimized phos-tag mobility shift assay for the detection of protein phosphorylation in planta
Journal of Plant Biotechnology
2018 .01
-
3,6-Anhydro-L-galactose Increases the Production of Hyaluronic Acid via Activation of ERK, Akt and AMPK Signaling Pathway in Human Keratinocytes
한국식품영양과학회 학술대회발표집
2018 .10
-
Morus alba L. root Reduces Melanin Synthesis via the S1P-S1PR-ERK Signaling Pathway in B16F10 Cells
한국식품영양과학회 학술대회발표집
2022 .10
-
Enhancing Plant Defense: The Synergy between Biochar and Ca2+ Signaling
한국토양비료학회 학술발표회 초록집
2024 .10
-
Development of Protein Biomarkers for the Authentication of Organic Rice
Journal of applied biological chemistry
2015 .01
-
Comparison of characteristics of soy protein based patties with different types of binding agents
한국식품영양과학회 학술대회발표집
2022 .10
-
In silico analysis of structure and RNA binding site of Potato virus Y coat protein by using homology model
한국농약과학회 학술발표대회 논문집
2018 .10
-
Krill oil inhibits angiogenesis by inhibiting VEGF-mediated eNOS/ERK/AKT signaling pathway
한국식품영양과학회 학술대회발표집
2022 .10
-
IQGAP1, a signaling scaffold protein, as a molecular target of a small molecule inhibitor to interfere with T cell receptor-mediated integrin activation
Korean Journal of Agricultural Science
2020 .06
이 논문의 저자 정보
최근 본 자료
댓글(0)
0