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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.1
- 수록면
- 727 - 736 (10page)
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초록· 키워드
오류제보하기
In this study, an immuno-magnetic bead (IMB)-based assay was developed to simultaneously detect Escherichia coli, Staphylococcus aureus, and Salmonella spp. and was tested in four animal-derived foods: beef, ham, egg, and ricotta cheese. The IMB-based assay exhibited good specificity by binding to five E. coli serotypes [capture efficiency (CE) average (avg.) 90.4%], five S. aureus strains (CE avg. 91.4%), and five Salmonella serotypes (CE avg. 95.4%) but not binding to non-target bacteria (CE<10%). Furthermore, the assay detected all three pathogens with a detection limit of 10 CFU/g without the need for enrichment or additional platforms. Since the results demonstrated that the IMB-based assay can effectively separate and enrich target bacteria from a variety of animal-derived food matrixes, the assay exhibits good specificity for potential use in providing rapid, immunological, presumptive identification of pathogenic bacteria.
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참고문헌 (28)
참고문헌 신청
Amaro M / 2012 / Scano-magneto immunoassay based on carbon nanotubes/gold nanoparticles nanocomposite for Salmonella enterica serovar Typhimurium detection / Biosens Bioelectron 38:157~162
Aydin M / 2014 / Rapid and sensitive detection of Escherichia coli O157:H7 in milk and ground beef using magnetic bead-based immunoassay coupled with tyramide signal amplification / J Food Prot 77:100~105
Benoit PW / 2003 / Methods for rapid separation and concentration of bacteria in food that bypass timeconsuming cultural enrichment / J Food Prot 66:1935~1948
Centers for Disease Control and Prevention / Hepatitis C information for health professionals. Guidelines for laboratory testing and result reporting
Chan KY / 2013 / Ultrasensitive detection of E. coli O157:H7 with biofunctional magnetic bead concentration via nanoporous membrane based electrochemical immunosensor / Biosens Bioelectron 41:532~537
Aydin M / 2014 / Rapid and sensitive detection of Escherichia coli O157:H7 in milk and ground beef using magnetic bead-based immunoassay coupled with tyramide signal amplification / J Food Prot 77:100~105
Benoit PW / 2003 / Methods for rapid separation and concentration of bacteria in food that bypass timeconsuming cultural enrichment / J Food Prot 66:1935~1948
Centers for Disease Control and Prevention / Hepatitis C information for health professionals. Guidelines for laboratory testing and result reporting
Chan KY / 2013 / Ultrasensitive detection of E. coli O157:H7 with biofunctional magnetic bead concentration via nanoporous membrane based electrochemical immunosensor / Biosens Bioelectron 41:532~537
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