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논문 기본 정보

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학술저널
저자정보
Kim, Seoung-Cheol (Department of Microbiology, Institute for Immunology and Immunological Disease, Yonsei University College of Medicine) Jeon, Bo-Young (Department of Biomedical Laboratory Science, College of Health Science, Yonsei University) Kim, Jin-Sook (Food & Drug Administration) Choi, In Hwan (National Masan Tuberculosis Hospital) Kim, Jiro (Department of Biomedical Laboratory Science, College of Health Science, Yonsei University) Woo, Jeongim (Department of Biomedical Laboratory Science, College of Health Science, Yonsei University) Kim, Soojin (Department of Biomedical Laboratory Science, College of Health Science, Yonsei University) Lee, Hyeong Woo (Department of Biomedical Laboratory Science, College of Health Science, Yonsei University) Sezim, Monoldorova (Department of Biomedical Laboratory Science, College of Health Science, Yonsei University) Cho, Sang-Nae (Department of Microbiology, Institute for Immunology and Immunological Disease, Yonsei University College of Medicine)
저널정보
대한결핵 및 호흡기학회 Tuberculosis and Respiratory Diseases 결핵 및 호흡기 질환 제79권 제4호
발행연도
2016.1
수록면
282 - 288 (7page)

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Background: Tuberculosis (TB) is a major health problem, and accurate and rapid diagnosis of multidrug-resistant (MDR) and extended drug-resistant (XDR) TB is important for appropriate treatment. In this study, performances of solid and liquid culture methods were compared with respect to MDR- and XDR-TB isolate recovery and drug susceptibility testing. Methods: Sputum specimens from 304 patients were stained with Ziehl-Neelsen method. Mycobacterium tuberculosis (Mtb) isolates were tested for recovery on $L{\ddot{o}wenstein$-Jensen (LJ) medium and the BacT Alert 3D system. For drug susceptibility testing of Mtb, isolates were evaluated on M-KIT plates and the BacT Alert 3D system. Results: The recovery rates were 94.9% (206/217) and 98.2% (213/217) for LJ medium and the BacT Alert 3D system, respectively (kappa coefficient, 0.884). The rate of drug resistance was 13.4% for at least one or more drugs, 6.0% for MDR-TB and 2.3% for XDR-TB. M-KIT plate and BacT 3D Alert 3D system were comparable in drug susceptibility testing for isoniazid (97.7%; kappa coefficient, 0.905) and rifampin (98.6%; kappa coefficient, 0.907). Antibiotic resistance was observed using M-KIT plates for 24 of the total 29 Mtb isolates (82.8%). Conclusion: The liquid culture system showed greater reduction in the culture period, as compared with LJ medium; however, drug susceptibility testing using M-KIT plates was advantageous for simultaneous testing against multiple drug targets.

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