지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.12
- 수록면
- 215 - 219 (5page)
이용수
초록· 키워드
오류제보하기
The problems of tuberculosis and its drug resistance are very severe. Therefore, rapid and accurate drug susceptibility assay is required. Recently, there has been an increased understanding of the genetic mechanism of Mycobacterium tuberculosis (MTB) drug resistance as well as advancement of molecular technologies. While many gene mutations correlate well with drug resistance, many genes do not show a strong correlation with drug resistance. For this reason, the current study assessed the utility of rpoB mRNA as a target to detect live mycobacteria. In this study, RT-PCR targeting of rpoB mRNA in BCG treated with rifampin was performed. Conventional RT-PCR and real-time PCR targeting rpoB mRNA as well as 85B mRNA was performed to determine whether these two methods could distinguish between viable and non-viable MTB. The levels of rpoB and 85B mRNA detected by RT- PCR were compared in parallel with colony forming unit counts of BCG that were treated with rifampin for different periods of time. The data suggests that that even though both mRNA levels of rpoB and 85B decreased gradually when rifampin-treatment increased, the rpoB mRNA seemed to represent live bacteria better than 85B mRNA. This study clearly indicates that RT-PCR is a good method to monitor viable cell counts in the liquid culture treated with the anti-tuberculosis drug.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS AND DISCUSSION
REFERENCES
참고문헌 (21)
참고문헌 신청
[학술지(정기간행물)] - Banaiee N / 2001 / Luciferase reporter mycobacteriophages for detection, identification, and antibiotic susceptibility testing of Mycobacterium tuberculosis in Mexico / J Clin Microbiol 39 : 3883 ~ 3888
[학술지(정기간행물)] - Centers for Disease Control and Prevention / 2007 / Extensively drugresistant tuberculosis-United States / MMWR 56 : 250 ~ 253
[학술지(정기간행물)] - 조장은 / 2014 / RpoB127-135 Peptide Derived from Mycobacterium tuberculosis is Processed and Presented to HLA-A*0201 Restricted CD8+ T Cells via an Alternate HLA-I Processing Pathway / 대한의생명과학회지 20 (4) : 250 ~ 255
[학술지(정기간행물)] - Combs DL / 1990 / USPHS tuberculosis shortcourse chemotherapy trial 21: effectiveness, toxicity, and acceptability / Ann Intern Med 112 : 397 ~ 406
[학술지(정기간행물)] - Desjardin LE / 1998 / Comparison of the ABI 7700 System (TaqMan) and competitive PCR for quantification of IS6110 DNA in sputum during treatment of tuberculosis / J Clin Microbiol 36 : 1964 ~ 1968
[학술지(정기간행물)] - Centers for Disease Control and Prevention / 2007 / Extensively drugresistant tuberculosis-United States / MMWR 56 : 250 ~ 253
[학술지(정기간행물)] - 조장은 / 2014 / RpoB127-135 Peptide Derived from Mycobacterium tuberculosis is Processed and Presented to HLA-A*0201 Restricted CD8+ T Cells via an Alternate HLA-I Processing Pathway / 대한의생명과학회지 20 (4) : 250 ~ 255
[학술지(정기간행물)] - Combs DL / 1990 / USPHS tuberculosis shortcourse chemotherapy trial 21: effectiveness, toxicity, and acceptability / Ann Intern Med 112 : 397 ~ 406
[학술지(정기간행물)] - Desjardin LE / 1998 / Comparison of the ABI 7700 System (TaqMan) and competitive PCR for quantification of IS6110 DNA in sputum during treatment of tuberculosis / J Clin Microbiol 36 : 1964 ~ 1968
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UCI(KEPA) : I410-ECN-0101-2017-510-002026448