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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.1
- 수록면
- 171 - 176 (6page)
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초록· 키워드
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Streptomyces avermitilis produces clinically useful drugs such as avermectins and oligomycins. Its genome contains approximately 33 cytochrome P450 genes and they seem to play important roles in the biosynthesis of many secondary metabolites. The SAV_7130 gene from S. avermitilis encodes CYP158A3. The amino acid sequence of this enzyme has high similarity with that of CYP158A2, a biflaviolin synthase from S. coelicolor A3(2). Recombinant S. avermitilis CYP158A3 was heterologously expressed and purified. It exhibited the typical P450 Soret peak at 447 nm in the reduced CO-bound form. Type I binding spectral changes were observed when CYP158A3 was titrated with myristic acid; however, no oxidative product was formed. An analog of flaviolin, 2-hydroxynaphthoquinone (2-OH NQ) displayed similar type I binding upon titration with purified CYP158A3. It underwent an enzymatic reaction forming dimerized product. A homology model of CYP158A3 was superimposed with the structure of CYP158A2, and the majority of structural elements aligned. These results suggest that CYP158A3 might be an orthologue of biflaviolin synthase, catalyzing C-C coupling reactions during pigment biosynthesis in S. avermitilis.
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참고문헌 (22)
참고문헌 신청
Burg, R. W. / 1979 / Avermectins, new family of potent anthelmintic agents: producing organism and fermentation / Antimicrob. Agents Chemother 15:361~367
Demain, A. L. / 1999 / Pharmaceutically active secondary metabolites of microorganisms / Appl. Microbiol. Biotechnol 52:455~463
Guengerich, F. P. / 2008 / Cytochrome p450 and chemical toxicology / Chem. Res. Toxicol 21:70~83
Han, S. / 2015 / Functional characterization of CYP107W1 from Streptomyces avermitilis and biosynthesis of macrolide oligomycin A / Arch. Biochem. Biophys 575:1~7
Songhee HAN / 2016 / Structural Analysis of the Streptomyces avermitilis CYP107W1-Oligomycin A Complex and Role of the Tryptophan 178 Residue / Molecules and Cells 39(3):211~216
Demain, A. L. / 1999 / Pharmaceutically active secondary metabolites of microorganisms / Appl. Microbiol. Biotechnol 52:455~463
Guengerich, F. P. / 2008 / Cytochrome p450 and chemical toxicology / Chem. Res. Toxicol 21:70~83
Han, S. / 2015 / Functional characterization of CYP107W1 from Streptomyces avermitilis and biosynthesis of macrolide oligomycin A / Arch. Biochem. Biophys 575:1~7
Songhee HAN / 2016 / Structural Analysis of the Streptomyces avermitilis CYP107W1-Oligomycin A Complex and Role of the Tryptophan 178 Residue / Molecules and Cells 39(3):211~216
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