지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
- 수록면
- 501 - 509 (9page)
이용수
초록· 키워드
오류제보하기
Shikonin, which derives from Lithospermum erythrorhizon, has been traditionally used against a variety of diseases, including cancer, in Eastern Asia. Here we determined that shikonin inhibits proliferation of gastric cancer cells by inducing apoptosis. Shikonin's biological activity was validated by observing cell viability, caspase 3 activity, reactive oxygen species (ROS) generation, and apoptotic marker expressions in AGS stomach cancer cells. The concentration range of shikonin was 35-250 nM with the incubation time of 6 h. Protein levels of Nrf2 and p53 were evaluated by western blotting and confirmed by real-time PCR. Our results revealed that shikonin induced the generation of ROS as well as caspase 3-dependent apoptosis. c-Jun-N-terminal kinases (JNK) activity was significantly elevated in shikonin-treated cells, thereby linking JNK to apoptosis. Furthermore, our results revealed that shikonin induced p53 expression but repressed Nrf2 expression. Moreover, our results suggested that there may be a co-regulation between p53 and Nrf2, in which transfection with siNrf2 induced the p53 expression. We demonstrated for the first time that shikonin activated cell apoptosis in AGS cells via caspase 3- and JNK-dependent pathways, as well as through the p53-Nrf2 mediated signal pathway. Our study validates in partly the contribution of shikonin as a new therapeutic approaches/agent for cancer chemotherapy.
목차
등록된 정보가 없습니다.
참고문헌 (32)
참고문헌 신청
Akhdar, H. / 2009 / Involvement of Nrf2 activation in resistance to 5-fluorouracil in human colon cancer HT-29 cells / Eur. J. Cancer 45:2219~2227
Alam, J. / 1999 / Nrf2, a Cap'n'Collar transcription factor, regulates induction of the heme oxygenase-1 gene / J. Biol. Chem 274:26071~26078
Chen, X. / 2002 / Cellular pharmacology studies of shikonin derivatives / Phytother. Res 16:199~209
Chu, F. F. / 2004 / Role of Se-dependent glutathione peroxidases in gastrointestinal inflammation and cancer / Free Radic. Biol. Med 36:1481~1495
Devasagayam, T. P. / 2004 / Free radicals and antioxidants in human health: current status and future prospects / J. Assoc. Physicians India 52:794~804
Alam, J. / 1999 / Nrf2, a Cap'n'Collar transcription factor, regulates induction of the heme oxygenase-1 gene / J. Biol. Chem 274:26071~26078
Chen, X. / 2002 / Cellular pharmacology studies of shikonin derivatives / Phytother. Res 16:199~209
Chu, F. F. / 2004 / Role of Se-dependent glutathione peroxidases in gastrointestinal inflammation and cancer / Free Radic. Biol. Med 36:1481~1495
Devasagayam, T. P. / 2004 / Free radicals and antioxidants in human health: current status and future prospects / J. Assoc. Physicians India 52:794~804
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
구강편평상피세포암종에서 shikonin에 의한 상피간엽이행 억제
대한구강해부학회지
2019 .01
-
Shikonin Induced Necroptosis via Reactive Oxygen Species in the T-47D Breast Cancer Cell Line
Asian Pacific journal of cancer prevention : APJCP
2015 .01
-
Shikonin Exerts Cytotoxic Effects in Human Colon Cancers by Inducing Apoptotic Cell Death via the Endoplasmic Reticulum and Mitochondria-Mediated Pathways
Biomolecules & Therapeutics(구 응용약물학회지)
2019 .01
-
Mechanism Underlying Shikonin-induced Apoptosis and Cell Cycle Arrest on SCC25 Human Tongue Squamous Cell Carcinoma Cell Line
International Journal of Oral Biology
2015 .01
-
The Inhibitory Effect of Shikonin on the Agonist-Induced Regulation of Vascular Contractility
Biomolecules & Therapeutics(구 응용약물학회지)
2015 .01
-
Shikonin Isolated from Lithospermum erythrorhizon Downregulates Proinflammatory Mediators in Lipopolysaccharide-Stimulated BV2 Microglial Cells by Suppressing Crosstalk between Reactive Oxygen Species and NF-κB
Biomolecules & Therapeutics(구 응용약물학회지)
2015 .01
-
Overexpression of Nrf2 promotes colon cancer progression via ERK and AKT signaling pathways
Annals of Surgical Treatment and Research
2020 .04
-
Dysregulation of NRF2 in Cancer: from Molecular Mechanisms to Therapeutic Opportunities
Biomolecules & Therapeutics(구 응용약물학회지)
2018 .01
-
Oxidative Damage and Nuclear Factor Erythroid 2-Related Factor 2 Protein Expression in Normal Skin and Keloid Tissue
Annals of Dermatology
2015 .01
-
Nrf2 expression by bisphenol A and its substitutes regulates human B cell survival
환경독성보건학회 심포지엄 및 학술대회
2021 .10
-
Molecular Mechanisms of Apoptosis and Roles in Cancer Development and Treatment
Asian Pacific journal of cancer prevention : APJCP
2015 .01
-
MicroRNA-based diagnosis and therapy in NRF2-stabilized tumor
대한동의병리학회 학술대회논문집
2015 .10
-
MS-5, a Naphthalene Derivative, Induces the Apoptosis of an Ovarian Cancer Cell CAOV-3 by Interfering with the Reactive Oxygen Species Generation
Biomolecules & Therapeutics(구 응용약물학회지)
2019 .01
-
Impact of Nrf2 overexpression on cholangiocarcinoma treatment and clinical prognosis
KOREAN JOURNAL OF CLINICAL ONCOLOGY
2023 .06
-
저칼륨혈증 흰쥐 신장에서 Nrf2와 p-Nrf2 단백발현의 변화
해부·생물인류학
2015 .03
-
Induction of Apoptosis by Eugenol and Capsaicin in Human Gastric Cancer AGS Cells - Elucidating the Role of p53
Asian Pacific journal of cancer prevention : APJCP
2015 .01
-
Anticancer activity and potential mechanisms of 1C, a ginseng saponin derivative, on prostate cancer cells
Journal of Ginseng Research
2018 .04
-
Association of a Methanol Extract of Rheum undulatum L. Mediated Cell Death in AGS Cells with an Intrinsic Apoptotic Pathway
Journal of Pharmacopuncture
2015 .01
-
The regulation of p62-Nrf2-Keap1 axis in dysregulation of lipid metabolism
대한동의병리학회 학술대회논문집
2015 .10
-
Propofol Attenuates Hypoxia/Reoxygenation-Induced Apoptosis and Autophagy in HK-2 Cells by Inhibiting JNK Activation
Yonsei Medical Journal
2019 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0