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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 43 - 49 (7page)
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Objectives : In this study, the anti-stress effects of ethanolic extract of Opuntiaficus-indica (OF70E) were investigated. Methods : To determine the effects of OF70E on physical stress, changes in whole-body cortisol level or behavior were monitored in zebrafish. After treatment with 0.9% saline or OF70E for 6 min, all fish underwent net handling stress (NHS), which induced physical stress. And then, we conducted open field test (OFT) or sacrificed fish for collecting body fluid from whole-body. We used the cortisol enzyme-linked immunoassay kit to measure the amount of cortisol in each zebrafish sample. Results : In result, compared with normal group which were not treated by NHS, whole-body cortisol levels were significantly increased in stressed-control group. Compared with control group, pretreatment with OF70E at concentrations of 25, 50 and 100 mg/L for 6min significantly inhibited the increase of whole-body cortisol levels induced by NHS(p<0.05). To anti-stress effects of OF70E on behavior, we conducted OFT after the induction of NHS following pretreatment of OF70E. As results of OFT, compared with unstressed-normal group, distance moved was significantly decreased by induction of NHS in stressed-control group (p<0.05). OF70E-pretreatment blocked decreases of distance moved increased by NHS (p<0.05). And meandering movement, immobility and turn angle were significantly increased by NHS in stressed-control group compared with unstressed-normal group (p<0.05). OF70E-pretreatment prevented the increases of meandering movement immobility and turn angle by NHS (p<0.05). Conclusions : In conclusion these results suggest that OF70E-pretreatment may prevent stress responses.
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참고문헌 (29)
참고문헌 신청
Andrade L / 2000 / Crossnational comparisons of the prevalences and correlates of mental disorders. WHO International Consortium in Psychiatric Epidemiology / Bull World Health Organ 78(4):413~426
Engert V / 2014 / Cortisol increase in empathic stress is modulated by emotional closeness and observation modality / Psychoneuroendocrinology 45:192~201
Cohen S / 1996 / Health psychology: psychological factors and physical disease from the perspective of human psychoneuroimmunology / Annu Rev Psychol 47:113~142
Brittijn SA / 2009 / Zebrafish development and regeneration: new tools for biomedical research / Int J Dev Biol 53(5-6):835~850
Spitsbergen JM / 2003 / The state of the art of the zebrafish model for toxicology and toxicologic pathology research--advantages and current limitations / Toxicol Pathol 31:62~87
Engert V / 2014 / Cortisol increase in empathic stress is modulated by emotional closeness and observation modality / Psychoneuroendocrinology 45:192~201
Cohen S / 1996 / Health psychology: psychological factors and physical disease from the perspective of human psychoneuroimmunology / Annu Rev Psychol 47:113~142
Brittijn SA / 2009 / Zebrafish development and regeneration: new tools for biomedical research / Int J Dev Biol 53(5-6):835~850
Spitsbergen JM / 2003 / The state of the art of the zebrafish model for toxicology and toxicologic pathology research--advantages and current limitations / Toxicol Pathol 31:62~87
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