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Subject

Antioxidant and anti-inflammatory activity of extracts from kohlrabi (Brassica Oleracea var. Gonglodes)
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콜라비 추출물의 항산화 및 항염 효능

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Type
Academic journal
Author
Yi, Mi-Ran (제주대학교 생명과학기술혁신센터) Kang, Chang-Hee (제주대학교 생명과학기술혁신센터) Bu, Hee-Jung (제주대학교 생명과학기술혁신센터)
Journal
한국유화학회 한국유화학회지 한국응용과학기술학회지 제34권 제2호
Published
2017.1
Pages
189 - 202 (14page)

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Antioxidant and anti-inflammatory activity of extracts from kohlrabi (Brassica Oleracea var. Gonglodes)
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This study was designed to examine the in vitro antioxidant and anti-inflammatory effects of kohlrabi (Brassica oleracea var. gonglodes) extract. Kohlrabi was extracted using 70% ethanol and then fractionated sequentially with n-hexane, ethyl acetate and butanol. Antioxidative ability was evaluated by bioassays using total polyphenol contents and ABTS (2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid diammonium salt) radical scavenging activity. Ethyl acetate fraction of kohlrabi was best on total polyphenol contents ($27.33{\pm}0.26mg\;GAE/g$) and ABTS radical scavenging effects ($IC_{50}\;172.9{\pm}1.6{\mu}g/mL$).For the anti-inflammatory activity in RAW 264.7 cells, the EtOAc fraction showed the highest inflammatory effect. Dose response studies were performed to determine the inhibitory effect of EtOAc fraction of kohlrabi on pro-inflammatory mediators in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. The EtOAc fraction of kohlrabi inhibited the NO and $PGE_2$ production and the protein level of iNOS and COX-2, and protein expression of pro-inflammatory cytokines (TNF-${\alpha}$, IL-6 and IL-$1{\beta}$), in a dose-dependent manner. These results suggest that kohlrabi has considerable potential as a ingredient with antioxidative and anti-inflammatory effects.

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