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논문 기본 정보

자료유형
학술저널
저자정보
Woo, Mi-Kyung (College of Pharmacy, Chung-Ang University) An, Jung-Mo (College of Pharmacy, Chung-Ang University) Kim, Jun-Dong (College of Pharmacy, Chung-Ang University) Park, Sue-Nie (National Institute of Toxicological Research) Kim, Hong-Jin (College of Pharmacy, Chung-Ang University)
저널정보
대한약학회 Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea 제31권 제2호
발행연도
2008.1
수록면
205 - 209 (5page)

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Cervical cancer caused by human papillomavirus (HPV) might be successfully prevented by HPV vaccination and screening. HPV vaccination and HPV serology assays have been investigated using HPV virus-like particles (VLPs). In this study we produced HPV18 L1 VLPs in Saccharomyces cerevisiae and purified them. The HPV18 L1 gene was cloned into the yeast expression vector $YEG{\alpha}-HIR525$, and transformed into Saccharomyces cereviaiae. Expression of HPV18 L1 protein was demonstrated by Western blotting. The HPV18 L1 protein was purified by ultracentrifugation, size-exclusion chromatography and cation-exchange chromatography, and was up to 95% pure. We showed by transmission electron microscopy that the purified protein self-assembled into VLPs. These findings should be useful for establishing vaccine efficacy as well as characterizing vaccine candidates, and may provide an international reference standard for HPV serology assays.

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